| Tag | Content |
|---|
| CPLM ID | CPLM-008742 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Flippase kinase 1 |
Protein Synonyms/Alias | |
Gene Name | FPK1 |
Gene Synonyms/Alias | YNR047W; N3449 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 525 | TNRVYALKVLSKDEM | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | Flippase activator that phosphorylates DFN1 and DFN2 and which is involved in the generation of phospholipid asymmetry in membranes by the inward translocation of phospholipids and in the retrieval pathway from early endosomes to the trans-Golgi network (TGN). Phosphorylates also the N-terminal half of YPK1. Involved in pheromone-response. |
Sequence Annotation | DOMAIN 496 777 Protein kinase.
DOMAIN 778 861 AGC-kinase C-terminal.
NP_BIND 502 510 ATP (By similarity).
ACT_SITE 621 621 Proton acceptor (By similarity).
BINDING 525 525 ATP (By similarity).
MOD_RES 140 140 Phosphoserine.
MOD_RES 144 144 Phosphoserine.
MOD_RES 171 171 Phosphoserine.
MOD_RES 175 175 Phosphoserine.
MOD_RES 185 185 Phosphoserine.
MOD_RES 300 300 Phosphoserine.
MOD_RES 414 414 Phosphoserine.
MOD_RES 462 462 Phosphoserine.
MOD_RES 674 674 Phosphothreonine.
MOD_RES 680 680 Phosphothreonine. |
Keyword | ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Kinase; Lipid transport; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Transport. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 893 AA |
Protein Sequence | MAGHHHEHEQ ERDHEQEHEH DSLQRPTTGS ERTRSISFSK LLTRSWKRNA SSSNNMSVSS 60
VNLYSDPENS RESDHNNSGS EGQSSRFSKL KSMFQSGNSS KNASAHNSSQ SSLEGDSASS 120
SSKLRYVKPM TSVANASPAS PPLSPTIPET DVLQTPKMVH IDQHEHEREH SNCGSPIMLS 180
SSSFSPTVAR TGTGRRRSPS TPIMPSQNSN NSSSTSAIRP NNYRHHSGSQ GFSSNNPFRE 240
RAGTVRSSNP YFAYQGLPTH AMSSHDLDEG FQPYANGSGI HFLSTPTSKT NSLTNTKNLS 300
NLSLNEIKEN EEVQEFNNED FFFHDIPKDL SLKDTLNGSP SRGSSKSPTI TQTFPSIIVG 360
FDNEYEEDNN NDKHDEKEEQ QTTTDNKTRN LSPTKQNGKA THPRIKIPLR RAASEPNGLQ 420
LASATSPTSS SARKTSGSSN INDKIPGQSV PPPNSFFPQE PSPKISDFPE PRRSRRLRTK 480
SFSNKFQDIM VGPQSFEKIR LLGQGDVGKV FLVREKKTNR VYALKVLSKD EMIKRNKIKR 540
VLTEQEILAT SNHPFIVTLY HSFQSEDYLY LCMEYCMGGE FFRALQTRKT KCICEDDARF 600
YASEVTAALE YLHLLGFIYR DLKPENILLH QSGHIMLSDF DLSIQAKDSK VPVVKGSAQS 660
TLVDTKICSD GFRTNSFVGT EEYIAPEVIR GNGHTAAVDW WTLGILIYEM LFGFTPFKGD 720
NTNETFTNIL KNEVSFPNNN EISRTCKDLI KKLLTKNESK RLGCKMGAAD VKKHPFFKKV 780
QWSLLRNQEP PLIPVLSEDG YDFAKLSSNK KRQTSQDSHK HLDEQEKNMF EERVEYDDEV 840
SEDDPFHDFN SMSLMEQDNN SMIYGNTNSY GKIAYTPNSN RSRSNSHRTF FKR 893 |
Gene Ontology | GO:0005737; C:cytoplasm; IDA:SGD. GO:0005886; C:plasma membrane; IDA:SGD. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0004674; F:protein serine/threonine kinase activity; IMP:SGD. GO:0006869; P:lipid transport; IEA:UniProtKB-KW. GO:0061092; P:positive regulation of phospholipid translocation; IGI:SGD. GO:0046777; P:protein autophosphorylation; IDA:SGD. GO:0019236; P:response to pheromone; IMP:SGD. |
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