CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003871
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Activated RNA polymerase II transcriptional coactivator p15 
Protein Synonyms/Alias
 Positive cofactor 4; PC4; SUB1 homolog; Single-stranded DNA-binding protein p9; p14 
Gene Name
 Sub1 
Gene Synonyms/Alias
 Pc4; Rpo2tc1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
3*****MPKSKELVSSacetylation[1]
5***MPKSKELVSSSSacetylation[1]
35KKQAVPEKPVKKQKPacetylation[2]
53SRALASSKQSSSSRDacetylation[3]
53SRALASSKQSSSSRDsuccinylation[3]
68DNMFQIGKMRYVSVRacetylation[4]
68DNMFQIGKMRYVSVRubiquitination[5]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA). 
Sequence Annotation
 REGION 2 50 Regulatory.
 REGION 77 101 Interaction with ssDNA (By similarity).
 MOD_RES 9 9 Phosphoserine.
 MOD_RES 10 10 Phosphoserine.
 MOD_RES 11 11 Phosphoserine.
 MOD_RES 12 12 Phosphoserine.
 MOD_RES 13 13 Phosphoserine.
 MOD_RES 15 15 Phosphoserine.
 MOD_RES 17 17 Phosphoserine.
 MOD_RES 19 19 Phosphoserine.
 MOD_RES 35 35 N6-acetyllysine (By similarity).
 MOD_RES 68 68 N6-acetyllysine (By similarity).
 MOD_RES 118 118 Phosphoserine (By similarity).  
Keyword
 Acetylation; Activator; Complete proteome; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 127 AA 
Protein Sequence
MPKSKELVSS SSSGSDSDSE VEKKLKRKKQ AVPEKPVKKQ KPGETSRALA SSKQSSSSRD 60
DNMFQIGKMR YVSVRDFKGK ILIDIREYWM DSEGEMKPGR KGISLNMEQW SQLKEQISDI 120
DDAVRKL 127 
Gene Ontology
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005667; C:transcription factor complex; IEA:Compara.
 GO:0003697; F:single-stranded DNA binding; IEA:Compara.
 GO:0003713; F:transcription coactivator activity; IEA:Compara.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR003173; PC4.
 IPR009044; ssDNA-bd_transcriptional_reg. 
Pfam
 PF02229; PC4 
SMART
  
PROSITE
  
PRINTS