CPLM-015786

Genbank Nucleotide ID

MOB kinase activator 1B

Protein Synonyms/Alias

Mob1 homolog 1A; Mob1A; Mob1B; Mps one binder kinase activator-like 1A

Homo sapiens (Human)

Position	Peptide	Type	References
17	SKTFKPKKNIPEGSH	ubiquitination	[1, 2, 3]
30	SHQYELLKHAEATLG	ubiquitination	[2, 3]
104	WADGTNIKKPIKCSA	ubiquitination	[2, 3, 4]
105	ADGTNIKKPIKCSAP	ubiquitination	[3]
142	KIGVPFPKNFMSVAK	ubiquitination	[2, 4, 5]
149	KNFMSVAKTILKRLF	acetylation	[6]

[1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]

Functional Description

Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L.

METAL 79 79 Zinc (By similarity).
METAL 84 84 Zinc (By similarity).
METAL 161 161 Zinc (By similarity).
METAL 166 166 Zinc (By similarity).
MOD_RES 2 2 N-acetylserine (By similarity).
MOD_RES 12 12 Phosphothreonine; by STK4/MST1.
MOD_RES 35 35 Phosphothreonine; by STK4/MST1.
MOD_RES 74 74 Phosphothreonine (By similarity).
MOD_RES 181 181 Phosphothreonine (By similarity).

Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Zinc.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0005634; C:nucleus; IDA:UniProtKB.
GO:0019209; F:kinase activator activity; IDA:UniProtKB.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0035329; P:hippo signaling cascade; IDA:UniProtKB.
GO:0046777; P:protein autophosphorylation; IDA:UniProtKB.

IPR005301; Mob1_phocein.

PF03637; Mob1_phocein