UniProt Accession

 HDGF_HUMAN; P51858; B3KU21; D3DVC9; Q5SZ07; Q5SZ08; Q5SZ09 

Genbank Protein ID

 D16431; AK096411; AL590666; CH471121; CH471121; CH471121; BC018991 

Genbank Nucleotide ID

 BAA03903.1; BAG53283.1; EAW52910.1; EAW52911.1; EAW52912.1; AAH18991.1 

Protein Name

 Hepatoma-derived growth factor 

Protein Synonyms/Alias

 HDGF; High mobility group protein 1-like 2; HMG-1L2 

Gene Name

 HDGF 

Gene Synonyms/Alias

 HMG1L2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
11	SNRQKEYKCGDLVFA	ubiquitination	[1, 2]
39	EMPEAAVKSTANKYQ	acetylation	[3]
39	EMPEAAVKSTANKYQ	ubiquitination	[4, 5, 6]
44	AVKSTANKYQVFFFG	acetylation	[7]
80	FGKPNKRKGFSEGLW	sumoylation	[8]
80	FGKPNKRKGFSEGLW	ubiquitination	[1, 2, 4, 5, 9]
96	IENNPTVKASGYQSS	sumoylation	[8]
96	IENNPTVKASGYQSS	ubiquitination	[5]
197	PLPMEVEKNSTPSEP	ubiquitination	[4]
226	DEEEEATKEDAEAPG	ubiquitination	[4, 5]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [8] SUMOylation of the hepatoma-derived growth factor negatively influences its binding to chromatin.
 Thakar K, Niedenthal R, Okaz E, Franken S, Jakobs A, Gupta S, Kelm S, Dietz F.
 FEBS J. 2008 Apr;275(7):1411-26. [PMID: 18331345]
 [9] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor. 

Sequence Annotation

 DOMAIN 12 69 PWWP.
 MOTIF 75 80 Nuclear localization signal.
 MOTIF 155 170 Bipartite nuclear localization signal.
 BINDING 19 19 Heparin (Probable).
 BINDING 21 21 Heparin (Probable).
 BINDING 72 72 Heparin (Probable).
 BINDING 75 75 Heparin (Probable).
 BINDING 79 79 Heparin (Probable).
 BINDING 80 80 Heparin (Probable).
 MOD_RES 44 44 N6-acetyllysine.
 MOD_RES 132 132 Phosphoserine.
 MOD_RES 133 133 Phosphoserine.
 MOD_RES 165 165 Phosphoserine.
 MOD_RES 199 199 Phosphoserine (By similarity).
 MOD_RES 200 200 Phosphothreonine.
 MOD_RES 202 202 Phosphoserine (By similarity).
 MOD_RES 206 206 Phosphoserine.
 MOD_RES 239 239 Phosphoserine.
 CROSSLNK 80 80 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Growth factor; Heparin-binding; Isopeptide bond; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 240 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; TAS:ProtInc.
 GO:0005615; C:extracellular space; IDA:BHF-UCL.
 GO:0017053; C:transcriptional repressor complex; IDA:BHF-UCL.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008201; F:heparin binding; TAS:ProtInc.
 GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
 GO:0001106; F:RNA polymerase II transcription corepressor activity; IDA:BHF-UCL.
 GO:0006987; P:activation of signaling protein activity involved in unfolded protein response; TAS:Reactome.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome. 

Interpro

 IPR000313; PWWP. 

Pfam

 PF00855; PWWP 

SMART

 SM00293; PWWP 

PROSITE

 PS50812; PWWP 

PRINTS