CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007070
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphoribosylformylglycinamidine synthase 
Protein Synonyms/Alias
 FGAM synthase; FGAMS; Formylglycinamide ribotide amidotransferase; FGARAT; Formylglycinamide ribotide synthetase 
Gene Name
 ADE6 
Gene Synonyms/Alias
 YGR061C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
116TISPWSSKATNIAHVubiquitination[1]
257NSEHCRHKIFNADWTubiquitination[1]
440KVLNHQGKEEIRGFHacetylation[2]
988KNLSKFEKILNENGVacetylation[2]
1038ELEQTWSKTSYEMQKubiquitination[1]
1081YNPADDMKIGLELSSubiquitination[3]
1263EGKATFSKSAEQLEKacetylation[2]
1307TNGIAGIKSPNGRVLubiquitination[1]
1336NSWYPEGKYEEWGGYubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
  
Sequence Annotation
 DOMAIN 1093 1358 Glutamine amidotransferase type-1.
 NP_BIND 345 356 ATP (Potential).
 ACT_SITE 1187 1187 For GATase activity (By similarity).  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Glutamine amidotransferase; Ligase; Nucleotide-binding; Purine biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1358 AA 
Protein Sequence
MTDYILPGPK ALSQFRVDNL IKDINSYTNS TSVINELRSC YIHYVNGIAQ NLSEQDTKLL 60
EVLLTYDSAL DIANDPLARQ LNDAVANNLP SSALGEDTYL IRVVPRSGTI SPWSSKATNI 120
AHVCGLQDKV QRIERGLALL IKTVPGFPLL ENLNDISLKC VYDRMTQQLY LTEPPNTMSI 180
FTHEEPKPLV HVPLTPKDTK QSPKDILSKA NTELGLALDS GEMEYLIHAF VETMKRDPTD 240
VELFMFAQVN SEHCRHKIFN ADWTIDGIKQ QFTLFQMIRN THKLNPEYTI SAYSDNAAVL 300
DSENDAFFFA PNSTTKEWTS TKERIPLLIK VETHNHPTAV SPFPGAATGS GGEIRDEGAT 360
GRGSKTKCGL SGFSVSDLLI PGNEQPWELN IGKPYHIASA LDIMIEAPLG SAAFNNEFGR 420
PCINGYFRTL TTKVLNHQGK EEIRGFHKPI MIAGGFGTVR PQFALKNTPI TPGSCLIVLG 480
GQSMLIGLGG GAASSVASGE GSADLDFASV QRGNPEMERR CQQVIDACVA LGNNNPIQSI 540
HDVGAGGLSN ALPELVHDND LGAKFDIRKV LSLEPGMSPM EIWCNESQER YVLGVSPQDL 600
SIFEEICKRE RAPFAVVGHA TAEQKLIVED PLLKTTPIDL EMPILFGKPP KMSRETITEA 660
LNLPEANLSE IPSLQDAIQR VLNLPSVGSK SFLITIGDRS VTGLIDRDQF VGPWQVPVAD 720
VGVTGTSLGE TIISTGEAMA MGEKPVNALI SASASAKLSV AESLLNIFAA DVKSLNHIKL 780
SANWMSPASH QGEGSKLYEA VQALGLDLCP ALGVAIPVGK DSMSMKMKWD DKEVTAPLSL 840
NITAFAPVFN TSKTWTPLLN RNTDDSVLVL VDLSAKQETK SLGASALLQV YNQVGNKSPT 900
VYDNAILKGF LESLIQLHQQ KEDIVLAYHD RSDGGLLITL LEMAFASRCG LEINIDGGDL 960
ESQLTNLFNE ELGAVFQISA KNLSKFEKIL NENGVAKEYI SIVGKPSFQS QEIKIINSTT 1020
NDVIYANSRS ELEQTWSKTS YEMQKLRDNP KTAEEEFASI TDDRDPGLQY ALTYNPADDM 1080
KIGLELSSQR PKVAILREQG VNGQMEMAWC FQQAGFNSVD VTMTDLLEGR FHLDDFIGLA 1140
ACGGFSYGDV LGAGAGWAKS VLYHEGVRSQ FSKFFNERQD TFAFGACNGC QFLSRLKDII 1200
PGCENWPSFE RNVSEQYEAR VCMVQISQEK DNSSEESVFL NGMAGSKLPI AVAHGEGKAT 1260
FSKSAEQLEK FEKDGLCCIR YVDNYGNVTE RFPFNPNGST NGIAGIKSPN GRVLAMMPHP 1320
ERVCRLEANS WYPEGKYEEW GGYGPWIRLF RSARRWVG 1358 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004642; F:phosphoribosylformylglycinamidine synthase activity; IMP:SGD.
 GO:0006189; P:'de novo' IMP biosynthetic process; IC:SGD.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW. 
Interpro
 IPR010918; AIR_synth_C_dom.
 IPR000728; AIR_synth_N_dom.
 IPR017926; GATASE.
 IPR010073; PRibForGlyAmidine_synth.
 IPR016188; PurM_N-like. 
Pfam
 PF00586; AIRS
 PF02769; AIRS_C 
SMART
  
PROSITE
 PS51273; GATASE_TYPE_1 
PRINTS