| Tag | Content |
|---|
| CPLM ID | CPLM-002797 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Bifunctional protein PutA |
Protein Synonyms/Alias | Proline dehydrogenase; Proline oxidase; Delta-1-pyrroline-5-carboxylate dehydrogenase; P5C dehydrogenase |
Gene Name | putA |
Gene Synonyms/Alias | poaA; b1014; JW0999 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 126 | PVAEQAHKLAYQLAD | acetylation | [1, 2] | | 134 | LAYQLADKLRNQKNA | acetylation | [2] | | 188 | RDALIRDKISNGNWQ | acetylation | [2] | | 604 | QTGLPHPKIPLPRDL | acetylation | [1, 2] | | 935 | EIADHTLKMLRGAMA | acetylation | [1, 2] | | 1128 | TLARQDAKYPVDAQL | acetylation | [2] | | 1230 | ALHRQLVKALPSAVS | acetylation | [1] | | 1244 | SERIQLAKAENITAQ | acetylation | [1] |
|
Reference | [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli. Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z. J Proteome Res. 2013 Feb 1;12(2):844-51. [ PMID: 23294111] [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Oxidizes proline to glutamate for use as a carbon and nitrogen source and also function as a transcriptional repressor of the put operon. |
Sequence Annotation | REGION 228 574 Proline dehydrogenase.
REGION 653 1119 Aldehyde dehydrogenase.
ACT_SITE 883 883 By similarity.
ACT_SITE 917 917 By similarity. |
Keyword | 3D-structure; Complete proteome; DNA-binding; FAD; Flavoprotein; Multifunctional enzyme; NAD; Oxidoreductase; Proline metabolism; Reference proteome; Repressor; Transcription; Transcription regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1320 AA |
Protein Sequence | MGTTTMGVKL DDATRERIKS AATRIDRTPH WLIKQAIFSY LEQLENSDTL PELPALLSGA 60
ANESDEAPTP AEEPHQPFLD FAEQILPQSV SRAAITAAYR RPETEAVSML LEQARLPQPV 120
AEQAHKLAYQ LADKLRNQKN ASGRAGMVQG LLQEFSLSSQ EGVALMCLAE ALLRIPDKAT 180
RDALIRDKIS NGNWQSHIGR SPSLFVNAAT WGLLFTGKLV STHNEASLSR SLNRIIGKSG 240
EPLIRKGVDM AMRLMGEQFV TGETIAEALA NARKLEEKGF RYSYDMLGEA ALTAADAQAY 300
MVSYQQAIHA IGKASNGRGI YEGPGISIKL SALHPRYSRA QYDRVMEELY PRLKSLTLLA 360
RQYDIGINID AEESDRLEIS LDLLEKLCFE PELAGWNGIG FVIQAYQKRC PLVIDYLIDL 420
ATRSRRRLMI RLVKGAYWDS EIKRAQMDGL EGYPVYTRKV YTDVSYLACA KKLLAVPNLI 480
YPQFATHNAH TLAAIYQLAG QNYYPGQYEF QCLHGMGEPL YEQVTGKVAD GKLNRPCRIY 540
APVGTHETLL AYLVRRLLEN GANTSFVNRI ADTSLPLDEL VADPVTAVEK LAQQEGQTGL 600
PHPKIPLPRD LYGHGRDNSA GLDLANEHRL ASLSSALLNS ALQKWQALPM LEQPVAAGEM 660
SPVINPAEPK DIVGYVREAT PREVEQALES AVNNAPIWFA TPPAERAAIL HRAAVLMESQ 720
MQQLIGILVR EAGKTFSNAI AEVREAVDFL HYYAGQVRDD FANETHRPLG PVVCISPWNF 780
PLAIFTGQIA AALAAGNSVL AKPAEQTPLI AAQGIAILLE AGVPPGVVQL LPGRGETVGA 840
QLTGDDRVRG VMFTGSTEVA TLLQRNIASR LDAQGRPIPL IAETGGMNAM IVDSSALTEQ 900
VVVDVLASAF DSAGQRCSAL RVLCLQDEIA DHTLKMLRGA MAECRMGNPG RLTTDIGPVI 960
DSEAKANIER HIQTMRSKGR PVFQAVRENS EDAREWQSGT FVAPTLIELD DFAELQKEVF 1020
GPVLHVVRYN RNQLPELIEQ INASGYGLTL GVHTRIDETI AQVTGSAHVG NLYVNRNMVG 1080
AVVGVQPFGG EGLSGTGPKA GGPLYLYRLL ANRPESALAV TLARQDAKYP VDAQLKAALT 1140
QPLNALREWA ANRPELQALC TQYGELAQAG TQRLLPGPTG ERNTWTLLPR ERVLCIADDE 1200
QDALTQLAAV LAVGSQVLWP DDALHRQLVK ALPSAVSERI QLAKAENITA QPFDAVIFHG 1260
DSDQLRALCE AVAARDGTIV SVQGFARGES NILLERLYIE RSLSVNTAAA GGNASLMTIG 1320 |
Gene Ontology | GO:0009898; C:internal side of plasma membrane; IDA:EcoCyc. GO:0003842; F:1-pyrroline-5-carboxylate dehydrogenase activity; IDA:EcoCyc. GO:0000986; F:bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding; IDA:EcoCyc. GO:0001141; F:bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; IDA:EcoCyc. GO:0050660; F:flavin adenine dinucleotide binding; IDA:EcoCyc. GO:0016620; F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor; IEA:InterPro. GO:0004657; F:proline dehydrogenase activity; IDA:UniProtKB. GO:0006537; P:glutamate biosynthetic process; IEA:InterPro. GO:0006561; P:proline biosynthetic process; IEA:InterPro. GO:0010133; P:proline catabolic process to glutamate; IMP:EcoCyc. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |