UniProt Accession

 GPH_ECOLI; P32662; Q2M750 

Genbank Protein ID

 Z19601; U18997; U00096; AP009048 

Genbank Nucleotide ID

 CAA79664.1; AAA58182.1; AAC76410.1; BAE77906.1 

Protein Name

 Phosphoglycolate phosphatase 

Protein Synonyms/Alias

 PGP; PGPase 

Gene Name

 gph 

Gene Synonyms/Alias

 yhfE; b3385; JW3348 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
78	TQRKTMGKPPVDDDI	acetylation	[1]
136	PLGLVTNKPTPFVAP	acetylation	[2]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Specifically catalyzes the dephosphorylation of 2- phosphoglycolate (2P-Gly). Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stress. 

Sequence Annotation

 REGION 13 15 Substrate (By similarity).
 ACT_SITE 13 13 Nucleophile (By similarity).
 METAL 13 13 Magnesium (By similarity).
 METAL 15 15 Magnesium (By similarity).
 METAL 192 192 Magnesium (By similarity).  

Keyword

 Carbohydrate metabolism; Chloride; Complete proteome; Hydrolase; Magnesium; Metal-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 252 AA 

Protein Sequence

Gene Ontology

 GO:0031404; F:chloride ion binding; IDA:EcoCyc.
 GO:0000287; F:magnesium ion binding; IDA:EcoCyc.
 GO:0008967; F:phosphoglycolate phosphatase activity; IDA:EcoCyc.
 GO:0006281; P:DNA repair; IMP:EcoCyc.
 GO:0046295; P:glycolate biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0019253; P:reductive pentose-phosphate cycle; IEA:HAMAP. 

Interpro

 IPR023214; HAD-like_dom.
 IPR006439; HAD-SF_hydro_IA_v1.
 IPR006402; HAD-SF_hydro_IA_v3.
 IPR005833; Haloacid_DH/epoxide_hydro.
 IPR006346; PGP_bact.
 IPR023198; PGP_dom2. 

Pfam

 PF00702; Hydrolase 

SMART

  

PROSITE

  

PRINTS

 PR00413; HADHALOGNASE.