| Tag | Content |
|---|
| CPLM ID | CPLM-006883 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Ubiquitin carboxyl-terminal hydrolase 14 |
Protein Synonyms/Alias | Deubiquitinating enzyme 14; Glucose-induced degradation protein 6; Ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14 |
Gene Name | UBP14 |
Gene Synonyms/Alias | GID6; YBR058C; YBR0515 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 132 | VPRTVLSKSTDSDIS | ubiquitination | [1] | | 370 | VYPDNNLKCQWIKLL | ubiquitination | [1] | | 398 | IKPTTFKKCIGQNHQ | ubiquitination | [1, 2] | | 500 | EGQTIEFKCANCKEK | ubiquitination | [1] | | 670 | LNPNLCRKALILNNG | ubiquitination | [1] | | 770 | ADSIEDMKKNGYIYF | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae. Starita LM, Lo RS, Eng JK, von Haller PD, Fields S. Proteomics. 2012 Jan;12(2):236-40. [ PMID: 22106047] |
Functional Description | Has a role in the negative regulation of gluconeogenesis. Required for proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase). Accelerates proteasomal breakdown of ubiquitinated proteins as it disassembles free ubiquitin chains that would compete with ubiquitinated proteins to bind to the proteasome. |
Sequence Annotation | DOMAIN 576 626 UBA 1.
DOMAIN 649 689 UBA 2.
ZN_FING 190 262 UBP-type.
ACT_SITE 332 332 Nucleophile.
ACT_SITE 737 737 Proton acceptor (By similarity). |
Keyword | Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Metal-binding; Nucleus; Protease; Reference proteome; Repeat; Thiol protease; Ubl conjugation pathway; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 781 AA |
Protein Sequence | MAEAVLENVN VPAVVSKDEC IYCFESPYNE PLALNASPKH SLNICLNCFQ ATCNRHVPLH 60
IRVTEYACDT IHSNYLTIAK VEKPKQENVE ENNNNKKIKL QVIETSEDDT HNTIWSLQRF 120
NGENVPRTVL SKSTDSDISS TALEKIEKIL KAKSQDFEDK KNSWVLEIST CPHTENFQIP 180
SKPENTVNLN QCSSCDLTQN LWLCLHCGNI GCGREQIGID GHSHALDHYR SNNNHPLAIK 240
LGSLSSSTYD LYCYACDDET RFPDNVNLGS ALQIYGINIQ EKIADEKTLV QLQVEQNENW 300
QFRMVDSSGK EFEKLSASKN YGCGLINLGN SCYLNSVIQS LVNGGVPNWS LDFLGSKFPL 360
DVVYPDNNLK CQWIKLLNAM KCEPELYPNG IKPTTFKKCI GQNHQEFSSN RQQDAMEFLT 420
FLLDLLDKKF FSSSSSGIPN PNDLVRFMME DRLQCNICGK VKYSYEPTEA IQIPLEENDE 480
PQDMLERIKA YFEGQTIEFK CANCKEKVTA NKKPGFKSLP QTLILNPIRI RLQNWIPVKT 540
SNELSLPGLI DRDDMLDVSS YLSQGFDPQT ENLLPDEDEN RSSFTPNQCS ISQLIEMGFT 600
QNASVRALFN TGNQDAESAM NWLFQHMDDP DLNDPFVPPP NVPKKDKREV DEVSLTSMLS 660
MGLNPNLCRK ALILNNGDVN RSVEWVFNNM DDDGTFPEPE VPNEEQQQKK DLGYSTAKPY 720
ALTAVICHKG NSVHSGHYVV FIRKLVADKW KWVLYNDEKL VAADSIEDMK KNGYIYFYTR 780
C 781 |
Gene Ontology | GO:0005737; C:cytoplasm; IC:SGD. GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. GO:0008242; F:omega peptidase activity; IEA:InterPro. GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro. GO:0004843; F:ubiquitin-specific protease activity; IDA:SGD. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:0045721; P:negative regulation of gluconeogenesis; IMP:SGD. GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |