CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022263
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Box C/D snoRNA protein 1 
Protein Synonyms/Alias
 Serologically defined breast cancer antigen NY-BR-75; Zinc finger HIT domain-containing protein 6 
Gene Name
 ZNHIT6 
Gene Synonyms/Alias
 BCD1; C1orf181 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
212INHPVGCKRKLAMSRubiquitination[1, 2, 3]
229TCGTEEAKYRCPRCMubiquitination[1, 2, 4]
246SCSLPCVKKHKAELTubiquitination[1, 2, 3, 4]
247CSLPCVKKHKAELTCubiquitination[2]
249LPCVKKHKAELTCNGubiquitination[2, 3, 4]
298ISRDAFLKRPISNKYubiquitination[2, 3, 5]
304LKRPISNKYMYFMKNubiquitination[1]
310NKYMYFMKNRARRQGubiquitination[1, 2]
321RRQGINLKLLPNGFTubiquitination[2]
329LLPNGFTKRKENSTFubiquitination[2, 3]
362SQAEYIEKRVPDDKTubiquitination[2, 3, 4, 5]
368EKRVPDDKTINEILKubiquitination[5]
375KTINEILKPYIDPEKubiquitination[2, 5]
382KPYIDPEKSDPVIRQubiquitination[2, 3]
424YYELDPYKSLLDNLRubiquitination[1, 2, 3, 5]
433LLDNLRNKVIIEYPTubiquitination[2]
453KGSNNDMKVLHQVKSubiquitination[2]
459MKVLHQVKSESTKNVubiquitination[1, 2]
464QVKSESTKNVGNEN*ubiquitination[4, 5]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Required for box C/D snoRNAs accumulation involved in snoRNA processing, snoRNA transport to the nucleolus and ribosome biogenesis. 
Sequence Annotation
 ZN_FING 220 254 HIT-type.
 MOD_RES 25 25 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome; Ribosome biogenesis; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 470 AA 
Protein Sequence
MEFAAENEGK SGGGLHSVAE GVRLSPEPGR EGVRDLAGAE EFGGGEEGTG LTGIKEIGDG 60
EEGSGQRPEE IPMDLTVVKQ EIIDWPGTEG RLAGQWVEQE VEDRPEVKDE NAGVLEVKQE 120
TDSSLVVKEA KVGEPEVKEE KVKEEVMDWS EVKEEKDNLE IKQEEKFVGQ CIKEELMHGE 180
CVKEEKDFLK KEIVDDTKVK EEPPINHPVG CKRKLAMSRC ETCGTEEAKY RCPRCMRYSC 240
SLPCVKKHKA ELTCNGVRDK TAYISIQQFT EMNLLSDYRF LEDVARTADH ISRDAFLKRP 300
ISNKYMYFMK NRARRQGINL KLLPNGFTKR KENSTFFDKK KQQFCWHVKL QFPQSQAEYI 360
EKRVPDDKTI NEILKPYIDP EKSDPVIRQR LKAYIRSQTG VQILMKIEYM QQNLVRYYEL 420
DPYKSLLDNL RNKVIIEYPT LHVVLKGSNN DMKVLHQVKS ESTKNVGNEN 470 
Gene Ontology
 GO:0070761; C:pre-snoRNP complex; IDA:BHF-UCL.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0000492; P:box C/D snoRNP assembly; IMP:BHF-UCL.
 GO:0042254; P:ribosome biogenesis; IEA:UniProtKB-KW. 
Interpro
 IPR007529; Znf_HIT. 
Pfam
 PF04438; zf-HIT 
SMART
  
PROSITE
 PS51083; ZF_HIT 
PRINTS