UniProt Accession

 E2F6_HUMAN; O75461; A8K2Z8; O60544; Q53QY9; Q7Z2H6 

Genbank Protein ID

 AF059292; AY083996; AY083997; AY083997; AJ493061; AK290413; AC099344; BC008348; BC107740; AF041381 

Genbank Nucleotide ID

 AAC31426.1; AAM10783.1; AAM10784.1; AAM10785.1; CAD37950.1; BAF83102.1; AAY14826.1; AAH08348.1; AAI07741.1; AAC14694.1 

Protein Name

 Transcription factor E2F6 

Protein Synonyms/Alias

 E2F-6 

Gene Name

 E2F6 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
118	DGIDLVEKKSKNHIR	ubiquitination	[1]
119	GIDLVEKKSKNHIRW	ubiquitination	[2]
141	FGAVPQQKKLQEELS	ubiquitination	[1]
142	GAVPQQKKLQEELSD	ubiquitination	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. 

Sequence Annotation

 DNA_BIND 50 129 Potential.
 REGION 130 222 Dimerization (Potential).
 REGION 143 164 Leucine-zipper.
 REGION 173 281 Transcription repression.
 MOTIF 95 129 DEF box.
 MOD_RES 67 67 Phosphoserine (By similarity).  

Keyword

 Alternative splicing; Cell cycle; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 281 AA 

Protein Sequence

Gene Ontology

 GO:0071339; C:MLL1 complex; IDA:UniProtKB.
 GO:0005667; C:transcription factor complex; IEA:InterPro.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:Compara.
 GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0000083; P:regulation of transcription involved in G1/S phase of mitotic cell cycle; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR015633; E2F.
 IPR003316; E2F_TDP.
 IPR015635; Transcription_factor_E2F6.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF02319; E2F_TDP 

SMART

  

PROSITE

  

PRINTS