CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021832
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein piccolo 
Protein Synonyms/Alias
 Aczonin; Multidomain presynaptic cytomatrix protein 
Gene Name
 Pclo 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
1338SDEKDLLKGLKKDSFubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking (By similarity). 
Sequence Annotation
 DOMAIN 4442 4536 PDZ.
 DOMAIN 4653 4752 C2 1.
 DOMAIN 4968 5059 C2 2.
 ZN_FING 523 547 C4-type (Potential).
 ZN_FING 1010 1033 C4-type (Potential).
 REGION 372 491 12 X 10 AA tandem approximate repeats of
 MOD_RES 617 617 Phosphoserine (By similarity).
 MOD_RES 756 756 Phosphoserine (By similarity).
 MOD_RES 873 873 Phosphothreonine (By similarity).
 MOD_RES 1133 1133 Phosphothreonine (By similarity).
 MOD_RES 1352 1352 Phosphoserine (By similarity).
 MOD_RES 1353 1353 Phosphoserine (By similarity).
 MOD_RES 1412 1412 Phosphoserine (By similarity).
 MOD_RES 1451 1451 Phosphoserine (By similarity).
 MOD_RES 1456 1456 Phosphoserine (By similarity).
 MOD_RES 1469 1469 Phosphoserine (By similarity).
 MOD_RES 1587 1587 Phosphoserine (By similarity).
 MOD_RES 1597 1597 Phosphoserine (By similarity).
 MOD_RES 1772 1772 Phosphothreonine (By similarity).
 MOD_RES 1775 1775 Phosphoserine (By similarity).
 MOD_RES 1778 1778 Phosphoserine (By similarity).
 MOD_RES 1784 1784 Phosphoserine (By similarity).
 MOD_RES 1820 1820 Phosphoserine (By similarity).
 MOD_RES 1840 1840 Phosphotyrosine (By similarity).
 MOD_RES 1841 1841 Phosphoserine (By similarity).
 MOD_RES 3014 3014 Phosphothreonine (By similarity).
 MOD_RES 3374 3374 Phosphoserine (By similarity).
 MOD_RES 3392 3392 Phosphothreonine (By similarity).
 MOD_RES 3529 3529 Phosphothreonine (By similarity).
 MOD_RES 3561 3561 Phosphoserine (By similarity).
 MOD_RES 3626 3626 Phosphoserine (By similarity).
 MOD_RES 3632 3632 Phosphoserine (By similarity).
 MOD_RES 3779 3779 Phosphotyrosine (By similarity).
 MOD_RES 3781 3781 Phosphoserine (By similarity).
 MOD_RES 3912 3912 Phosphothreonine (By similarity).
 MOD_RES 3913 3913 Phosphothreonine (By similarity).
 MOD_RES 3954 3954 Phosphoserine (By similarity).
 MOD_RES 3970 3970 Phosphoserine (By similarity).
 MOD_RES 4304 4304 Phosphoserine (By similarity).
 MOD_RES 4308 4308 Phosphoserine (By similarity).
 MOD_RES 4311 4311 Phosphoserine (By similarity).
 MOD_RES 4343 4343 Phosphoserine (By similarity).
 CARBOHYD 2702 2702 O-linked (GlcNAc) (By similarity).
 CARBOHYD 2976 2976 O-linked (GlcNAc) (By similarity).  
Keyword
 3D-structure; Alternative splicing; Calcium; Calcium/phospholipid-binding; Cell junction; Complete proteome; Glycoprotein; Metal-binding; Phosphoprotein; Reference proteome; Repeat; Synapse; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 5085 AA 
Protein Sequence
MGNEASLEGE GLPEGLAAAA GAGGSGSALH PGIPAGMEAD LSQLSEEERR QIAAVMSRAQ 60
GLPKGSVPPA AAESPSMHRK QELDSSQAPQ QPGKPPDPGR PTQPGLSKSR TTDTFRSEQK 120
LPGRSPSTIS LKESKSRTDF KEEYKSSMMP GFFSDVNPLS AVSSVVNKFN PFDLISDSEA 180
SQEETTKKQK VVQKEQGKSE GMAKPPLQQP SPKPIPKQQG QVKEVIQQDS SPKSVSSQQA 240
EKVKPQAPGT GKPSQQSPAQ TPAQQASPGK PVAQQPGSAK ATVQQPGPAK SPAQPAGTGK 300
SPAQPPAKTP GQQAGLEKTS SSQQPGPKSL AQTPGHGKFP LGPVKSPAQQ PGTAKHPAQQ 360
PGPQTAAKVP GPTKTPAQQS GPGKTPAQQP GPTKPSPQQP IPAKPQPQQP VATKTQPQQS 420
APAKPQPQQP APAKPQPQQP TPAKPQPQPP TPAKPQPQPP TATKPQPQPP TATKPHHQQP 480
GLAKPSAQQP TKSISQTVTG RPLQPPPTSA AQTPAQGLSK TICPLCNTTE LLLHIPEKAN 540
FNTCTECQST VCSLCGFNPN PHLTEIKEWL CLNCQMQRAL GGDLAAAIPS SPQPTPKAAT 600
APTATASKSP VPSQQASPKK EPPSKQDSPK ALESKKPPEP KKPPEPKKPP EPKKPPPLVK 660
QPTLHGPTPA TAPQLPVAEA LPEPAPPKEP SGPLPEQAKA PVGDVEPKQP KMTETRADIQ 720
SSSTTKPDIL SSQVQSQAQV KTASPLKTDS AKPSQSFPPT GEKTTPLDSK AMPRPASDSK 780
IISQPGPGSE SKDPKHIDPI QKKDEPKKAQ PKGSPKPETK PVPKGSPTPS GTRPTAGQAA 840
PPSQQPPKPQ EQSRRFSLNL GGITDAPKSQ PTTPQETVTG KLFGFGASIF SQASNLISTA 900
GQQGPHPQTG PAAPSKQAPT PSQSPAAQGP AKSTGQLPPA PAKATAVKKE AKAAAAENLE 960
SKPEQAPTAK KTEKDKKPPP AKVGKPPPSE PEKAVPAHKP DKTTKPKPAC PLCRTELNLG 1020
SQEPPNFNTC TECKNQVCNL CGFNPTPHLT EIQEWLCLNC QTQRAISGQL GDMGKMPPAP 1080
SGPKASPMPA PAEPSSQKTP TGTQVKGKKK EAEGKTEAEK PVPEKETASI EKTPPMVTTD 1140
QKLEESEGKK SKVSALPEKK PSEEEKAISA DKKERKPPAE EKPPLEEKKP IPVDKKLPPE 1200
AKPLSSEGEE KHEILKAHVQ IPEEEPTGKV AAKAGEEEQQ PDSRPEALPG ATPLTLPKAG 1260
EKERAVAQPQ AEGSSKDGQG ERSKEKTEKE EDKSDTSSSQ QPKSPQGLSD TGYSSDGISG 1320
SLGEIPSLIP SDEKDLLKGL KKDSFSQESS PSSPSDLAKL ESTVLSILEA QASTLVGEKA 1380
EKKTQPQKIS PEKPQDQQKT QTASETLDIT ISEEEIKESQ EKKVSPKKDS EQGFPSRKEH 1440
KEKPELVDDL SPRRASYDSV EDSSESENSP VVRRKRRTSI GSSSSDEYKQ EDSQGSGEEE 1500
DFIRKQIIEM SADEDASGSE DEEFIRSQLK EISGVGESQK REEAKGKGKG VAGKHRRLTR 1560
KSSTSFDDDA GRRHSWHDED DETFDESPEL KFRETKSQES EELVVAGGGG LRRFKTIELN 1620
STIADKYSSE SSQKKTILYF DEEPELEMES LTDSPEDRSR GEGSSSLHAS SFTPGTSPTS 1680
VSSLDEDSDS SPSHKKGESK QQRKARHRSH GPLLPTIEDS SEEEELREEE ELLKEQEKQR 1740
ELEQQQRKSS SKKSKKDKDE LRAQRRRERP KTPPSNLSPI EDASPTEELR QAAEMEELHR 1800
SSCSEYSPSI ESDPEGFEIS PEKIIEVQKV YKLPAAVSLY SPTDEQSVMQ KEGVQKALKS 1860
AEEMYEEMMQ KPHKYKAFPA ANERDEVFEK EPLYGGMLIE DYIYESLVED TYNGSVDGSL 1920
LTRQEEQNGF MQQRGREQKV RLQEQIYDDP MQKISDLQKE FYELESLHSV VPQEDIVSSS 1980
YIIPESHEIV DLGSMVMSTS EEKKLLDADS AYEELMRRQQ VQVTDGSSPV QTTIGDDMAE 2040
STLDFDRVQD ASLTSSILSG ASLTDSTSSA TLSIPDVKIT QQFSAEELED EYVTDYTREI 2100
QDIIAHESLI LTYSEPSESA TSVPPSDTPS LTSSISSVCT TDSSSPVTTL DSLTTVYTEP 2160
ADVMTKFKDS EEISSTYFPG SIIDYPEDIS VSLDRTIMPE SRTNEDRIVL SFSGMAPSVV 2220
ESVGTKPERP QADTISTDLP ISEKDLIKGK KETGDGIILE VLDAYKDKRE ESEAELTKIS 2280
LPEPGLAQAP SSVTAPQIKE QHVSPHSVSG KISGQEKPTY RLPSGSLPVS THPSKSRPFF 2340
RSSSLDISAQ PPPPPPPPPP SPSTSSPPPT PPLPPATSPK PPTYPKKKLA VAATVTSTTI 2400
VTTHVDALTM VEAAAARRSN GLPATKMCAI APPPVPPKPS QIPTGLVFTH RPEAIKPPIA 2460
PKPAVPQIPV TTQKPTDTCP KPTGLSLTST MSLNLVTSAD YNVPSPTSPL SPHSNKSSPR 2520
YSKSLMDTYV VITLPSEPGT PTDSSAAQAI TSWPLGSPPK DLVSLETVFS VVPPMTSTEI 2580
PSASQPTLYT SGALGTFSVT PAVTASLFQT VPTSLTQFLP AEASKPEVSA VSSAVPSVAP 2640
RSVSIPIPPE PLALDRHQYK ENGKLPLIGD AIDLRTIPKS EVKVTEKCMD LSASAMDVKR 2700
QTTANEVYRR QISAVQPSII NLSAASSLGT PVTMDSKTVA VVTCTDTTIY TTGTESQVGI 2760
EHAVTSPLQL TTSKHTELPY RKPSSQAFPT IRDEAPINLS LGPSAQAVTL AVTKPVTVPP 2820
VGVTNGWTDS TLSQGVADGE VVDLSTSKSH RTVVTMDEST SNVVTKIIED DEKPVDLTAG 2880
RRAVCCDMVY TLPFGRSCTA QQPATTLPED RFGYRDDHYQ YDRSGPYGYR GIGGMKPSMS 2940
DTNLPEAGHF FYKSKNAFDY SGGTGAAVDL TSGRVSTGEV MDYSSKTTGP YPETRQVISG 3000
VGISTPQYST ARLTPPPGPQ YGVGSVLRSS NGVVYSSVAT PIPSTFAITT QPGSIFSTTV 3060
RDLSGIPTTD AMTSLSALHQ SQPMPRSYFI TTGASETDIA VTGIDINASL QTITMETLPA 3120
ETMDSVPTLT TASEVFSEVV GEESTLLIVP DEDKQQQQLD LERELLELEK IKQQRFAEEL 3180
EWERQEIQRF REQEKIMVQK KLEELQSMKQ HLLYQQEEER QAQFMMRQET LAQQQLQLEQ 3240
IQQLQQQLHQ QLEEQKLRQI YQYNYDPSGT SSPQTTTEQA ILEGQYAATE GSQFWATEDA 3300
TTTASTVVAI EIPQSQGWYT VQSDGVTQYI APPGILSTVS EIPLTDVVVK EEKQPKKRSS 3360
GAKVRGQYDE MGESVADDPR NLKKIVDSGV QTDDEETADR SYASRRRRTK KSVDTSVQTD 3420
DEDQDEWDMP SRSRRKARTG KYGDSTAEGD KTKPLSKVSS VAVQTVAEIS VQTEPVGTIR 3480
TPSIRARVDA KVEIIKHISA PEKTYKGGSL GCQTETDSDT QSPPYLGATS PPKDKKRPTP 3540
LEIGYSSSHL RADPTVQLAP SPPKSPKVLY SPISPLSPGN ALEPAFVPYE KPLPDDISPQ 3600
KVLHPDMAKV PPASPKTAKM MQRSMSDPKP LSPTADESSR APFQYSEGFT TKGSQTMTAS 3660
GTQKKVKRTL PNPPPEEVST GTQSTYSTMG TASRRRMCRT NTMARAKILQ DIDRELDLVE 3720
RESAKLRKKQ AELDEEEKEI DAKLRYLEMG INRRKEALLK EREKRERAYL QGVAEDRDYM 3780
SDSEVSSTRP SRVESQHGVE RPRTAPQTEF SQFIPPQTQT EAQLVPPTSP YTQYQYSSPA 3840
LPTQAPTPYT QQSHFQQQTL YHQQVSPYQT QPTFQAVATM SFTPQAQPTP TPQPSYQLPS 3900
QMMVIQQKPR QTTLYLEPKI TSNYEVIRNQ PLMIAPVSTD NTYAVSHLGS KYNSLDLRIG 3960
LEERSSMAGS PISSISADSF YADIDHHTSR NYVLIDDIGD ITKGTAALST VFSLHEKDLS 4020
KTDRLLRTTE TRRSQEVTDF LAPLQTSSRL HSYVKADEDP MEDPYELKLL KHQIKQEFRR 4080
GTESLDHLAG LSHYYHADTS YRHFPKSEKY SISRLTLEKQ AAKQLPAAIL YQKQSKHKKS 4140
LIDPKMSKFS PIQESRDLEP DYPTYMSSGT SSIGGISSRA RLLQDDITFG LRKNITDQQK 4200
FMGSSLGSGL GTLGNTIRSA LQDEADKPYS SGSRSRPSSR PSSVYGLDLS IKRDSSSSSL 4260
RLKAQEAEAL DVSFGHSSSS ARTKPTSLPI SQSRGRIPIV AQSSEEESPL SPVGQPMGMA 4320
RAAAGPLPPI SADTRDQFGS SHSLPEVQQH MREESRTRGY DRDIAFIMDD FQHAMSDSEA 4380
YHLRREETDW FDKPRESRLE NGHGLDRKLP ERLVHSRPLS QHQEQILQMN GKTIHYIFPH 4440
ARVKITRDFK DHTGSGNGLG IRIVGGKEIP GHSGEIGAYI AKILPGESAE HTGPLMEGMQ 4500
VLEWNGVPLT SKTYEEVQSI INQQSGEAEI CVRLDLNMLS DSENPQHLEL HEPPKVDKAK 4560
SPGVDPKQLA AELQKVSLQQ SPLVMSSVVE KGSHAHSGPT SAGSSSVPSP GQPGSPSVSK 4620
KKHSSTKPTD GPKAASHPIT GEIQLQINYD LGNLIIHILQ ARNLVPRDNN GYSDPFVKVY 4680
LLPGRGQVMV VQNASAEYKR RTKYVQKSLN PEWNQTVIYK SISMEQLMKK TLEVTVWDYD 4740
RFSSNDFLGE VLIDLSSTSH LDNTPRWYPL KEQTESIDHG KSHSSQNSQQ SPKPSVIKSR 4800
SHGIFPDPSK DMQVPTIEKS HSSPGSSKSS SEGHLRSHGP SRSQSKTSVA QTHLEDAGVA 4860
IAAAEAAVQQ LRIQPTKPTN HRPAESSVST GSSGSSVGSG YSVDSEGSSC VAGEPNLLPI 4920
PRIGKMGQNG QDPVKQPGMG ATDTEGKTQV MGEIKLALKK EMKTDGEQLI VEILQCRNIT 4980
YKFKSPDHLP DLYVKLYVIN ISTQKKVIKK KTRVCRHDRE PSFNETFRFS LSPAGHSLQI 5040
LLFSNGGKFM KKTLIGEACI WLDKVDLRKR IVNWHKLLVS PTQTH 5085 
Gene Ontology
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0045202; C:synapse; IDA:UniProtKB.
 GO:0005509; F:calcium ion binding; IDA:UniProtKB.
 GO:0005544; F:calcium-dependent phospholipid binding; IDA:UniProtKB.
 GO:0005522; F:profilin binding; ISS:UniProtKB.
 GO:0007010; P:cytoskeleton organization; ISS:UniProtKB.
 GO:0016080; P:synaptic vesicle targeting; NAS:UniProtKB. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR001478; PDZ.
 IPR011011; Znf_FYVE_PHD.
 IPR008899; Znf_piccolo.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF00168; C2
 PF00595; PDZ
 PF05715; zf-piccolo 
SMART
 SM00239; C2
 SM00228; PDZ 
PROSITE
 PS50004; C2
 PS50106; PDZ 
PRINTS