UniProt Accession

 EIF3B_RAT; Q4G061 

Genbank Protein ID

 BC098728 

Genbank Nucleotide ID

 AAH98728.1 

Protein Name

 Eukaryotic translation initiation factor 3 subunit B 

Protein Synonyms/Alias

 eIF3b; Eukaryotic translation initiation factor 3 subunit 9; eIF-3-eta 

Gene Name

 Eif3b 

Gene Synonyms/Alias

 Eif3s9 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
192	KLKNVIHKIFSKFGK	acetylation	[1]
199	KIFSKFGKIINDYYP	acetylation	[1]
211	YYPEEDGKTKGYIFL	acetylation	[1]
237	VKNADGYKLDKQHTF	acetylation	[1]
349	LWGGDKFKQIQRFSH	acetylation	[1]
419	FKWSHDGKFFARMTL	acetylation	[1]
442	PSMGLLDKKSLKISG	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (By similarity). 

Sequence Annotation

 DOMAIN 168 251 RRM.
 REPEAT 315 353 WD 1.
 REPEAT 355 400 WD 2.
 REPEAT 404 442 WD 3.
 REPEAT 543 584 WD 4.
 REPEAT 632 677 WD 5.
 REGION 107 396 Sufficient for interaction with EIF3E (By
 REGION 153 257 Sufficient for interaction with EIF3J (By
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 31 31 Phosphothreonine (By similarity).
 MOD_RES 68 68 Phosphoserine (By similarity).
 MOD_RES 75 75 Phosphoserine (By similarity).
 MOD_RES 79 79 Phosphoserine (By similarity).
 MOD_RES 114 114 Phosphoserine.
 MOD_RES 117 117 Phosphoserine.
 MOD_RES 135 135 Phosphoserine (By similarity).
 MOD_RES 137 137 Phosphoserine (By similarity).
 MOD_RES 147 147 Phosphoserine (By similarity).
 MOD_RES 192 192 N6-acetyllysine (By similarity).
 MOD_RES 222 222 Phosphoserine (By similarity).
 MOD_RES 271 271 N6-acetyllysine (By similarity).
 MOD_RES 347 347 N6-acetyllysine (By similarity).
 MOD_RES 434 434 Phosphothreonine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome; Repeat; RNA-binding; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 797 AA 

Protein Sequence

Gene Ontology

 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; ISS:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; ISS:UniProtKB.
 GO:0006413; P:translational initiation; ISS:UniProtKB. 

Interpro

 IPR011042; 6-blade_b-propeller_TolB-like.
 IPR011400; EIF3B.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR013979; TIF_beta_prop-like.
 IPR015943; WD40/YVTN_repeat-like_dom. 

Pfam

 PF08662; eIF2A
 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS