CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004043
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ski oncogene 
Protein Synonyms/Alias
 Proto-oncogene c-Ski 
Gene Name
 SKI 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
227VYHECFGKCKGLLVPubiquitination[1]
294LSQDYTGKEEQARLGubiquitination[1, 2, 3]
318FDYGNKYKRRVPRVSacetylation[4]
335PPASIRPKTDDTSSQacetylation[4]
365TLAGSSNKSLGCVHPubiquitination[1, 5]
409RDSFYSYKSFETAVAubiquitination[2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling. 
Sequence Annotation
 MOD_RES 480 480 Phosphoserine.  
Keyword
 3D-structure; Coiled coil; Complete proteome; Nucleus; Phosphoprotein; Proto-oncogene; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 728 AA 
Protein Sequence
MEAAAGGRGC FQPHPGLQKT LEQFHLSSMS SLGGPAAFSA RWAQEAYKKE SAKEAGAAAV 60
PAPVPAATEP PPVLHLPAIQ PPPPVLPGPF FMPSDRSTER CETVLEGETI SCFVVGGEKR 120
LCLPQILNSV LRDFSLQQIN AVCDELHIYC SRCTADQLEI LKVMGILPFS APSCGLITKT 180
DAERLCNALL YGGAYPPPCK KELAASLALG LELSERSVRV YHECFGKCKG LLVPELYSSP 240
SAACIQCLDC RLMYPPHKFV VHSHKALENR TCHWGFDSAN WRAYILLSQD YTGKEEQARL 300
GRCLDDVKEK FDYGNKYKRR VPRVSSEPPA SIRPKTDDTS SQSPAPSEKD KPSSWLRTLA 360
GSSNKSLGCV HPRQRLSAFR PWSPAVSASE KELSPHLPAL IRDSFYSYKS FETAVAPNVA 420
LAPPAQQKVV SSPPCAAAVS RAPEPLATCT QPRKRKLTVD TPGAPETLAP VAAPEEDKDS 480
EAEVEVESRE EFTSSLSSLS SPSFTSSSSA KDLGSPGARA LPSAVPDAAA PADAPSGLEA 540
ELEHLRQALE GGLDTKEAKE KFLHEVVKMR VKQEEKLSAA LQAKRSLHQE LEFLRVAKKE 600
KLREATEAKR NLRKEIERLR AENEKKMKEA NESRLRLKRE LEQARQARVC DKGCEAGRLR 660
AKYSAQIEDL QVKLQHAEAD REQLRADLLR EREAREHLEK VVKELQEQLW PRARPEAAGS 720
EGAAELEP 728 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:BHF-UCL.
 GO:0016605; C:PML body; IDA:UniProtKB.
 GO:0005667; C:transcription factor complex; ISS:BHF-UCL.
 GO:0017053; C:transcriptional repressor complex; ISS:BHF-UCL.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0046811; F:histone deacetylase inhibitor activity; ISS:BHF-UCL.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003714; F:transcription corepressor activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IDA:UniProtKB.
 GO:0009948; P:anterior/posterior axis specification; ISS:BHF-UCL.
 GO:0030509; P:BMP signaling pathway; TAS:Reactome.
 GO:0060349; P:bone morphogenesis; ISS:BHF-UCL.
 GO:0048870; P:cell motility; NAS:BHF-UCL.
 GO:0008283; P:cell proliferation; NAS:BHF-UCL.
 GO:0030326; P:embryonic limb morphogenesis; ISS:BHF-UCL.
 GO:0060325; P:face morphogenesis; ISS:BHF-UCL.
 GO:0002089; P:lens morphogenesis in camera-type eye; ISS:BHF-UCL.
 GO:0022011; P:myelination in peripheral nervous system; ISS:BHF-UCL.
 GO:0014902; P:myotube differentiation; IDA:UniProtKB.
 GO:0032926; P:negative regulation of activin receptor signaling pathway; IDA:UniProtKB.
 GO:0030514; P:negative regulation of BMP signaling pathway; IDA:UniProtKB.
 GO:0048147; P:negative regulation of fibroblast proliferation; ISS:BHF-UCL.
 GO:0045668; P:negative regulation of osteoblast differentiation; IDA:UniProtKB.
 GO:0010626; P:negative regulation of Schwann cell proliferation; IGI:MGI.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IDA:UniProtKB.
 GO:0001843; P:neural tube closure; ISS:BHF-UCL.
 GO:0043585; P:nose morphogenesis; ISS:BHF-UCL.
 GO:0021772; P:olfactory bulb development; ISS:BHF-UCL.
 GO:0060021; P:palate development; ISS:BHF-UCL.
 GO:0043388; P:positive regulation of DNA binding; IDA:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; ISS:BHF-UCL.
 GO:0030177; P:positive regulation of Wnt receptor signaling pathway; NAS:BHF-UCL.
 GO:0070207; P:protein homotrimerization; IDA:UniProtKB.
 GO:0042981; P:regulation of apoptotic process; ISS:BHF-UCL.
 GO:0060041; P:retina development in camera-type eye; ISS:BHF-UCL.
 GO:0048741; P:skeletal muscle fiber development; ISS:BHF-UCL.
 GO:0060395; P:SMAD protein signal transduction; IDA:UniProtKB.
 GO:0035019; P:somatic stem cell maintenance; ISS:BHF-UCL.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; NAS:BHF-UCL. 
Interpro
 IPR014890; c-SKI_SMAD4-bd_dom.
 IPR009061; DNA-bd_dom_put.
 IPR010919; SAND_dom-like.
 IPR003380; Transform_Ski.
 IPR023216; Tscrpt_reg_SKI_SnoN. 
Pfam
 PF08782; c-SKI_SMAD_bind
 PF02437; Ski_Sno 
SMART
 SM01046; c-SKI_SMAD_bind 
PROSITE
  
PRINTS