CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-038571
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Mitochondrial inner membrane protein 
Protein Synonyms/Alias
  
Gene Name
 Immt 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
178KAHSNILKTAMDNSEacetylation[1]
190NSEIAGEKKSAQWRTubiquitination[2]
203RTVEGALKERRKAVDacetylation[3, 4]
218EAADALLKAKEELEKacetylation[4]
220ADALLKAKEELEKMKacetylation[4]
227KEELEKMKTIIEDAKacetylation[4]
357HIELALEKHKLEEKRacetylation[4]
359ELALEKHKLEEKRTFacetylation[4]
427KVQEQELKYEFEQGLacetylation[4, 5, 6]
437FEQGLSEKLSEQELEubiquitination[2]
561ARFYAVQKLARRVAMacetylation[4]
596LFPPKQLKPPAELYPacetylation[1, 4, 7]
635AKFVNQLKGESRRVAacetylation[7]
647RVAQDWLKEARMTLEacetylation[7]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [7] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 679 AA 
Protein Sequence
MLRACQLSGV TVAAQSCLCG KFVLRPLRPC RRYSTSSSSG LTAGKIAGAG LLFVGGGIGG 60
TILYAKWDSH FRESVEKTIP YSDKLFGMVL GSAPYTVPLP KKPVQSGPLK ISSVSEVMKD 120
SKLPVAQSQK TKGDTPASAA LAKSLEDALN RTSSVTLQTI TAQNAAVQAV KAHSNILKTA 180
MDNSEIAGEK KSAQWRTVEG ALKERRKAVD EAADALLKAK EELEKMKTII EDAKKREIAG 240
ATPHITAAEG RLHNMIVDLD NVVKKVQAAQ SEAKVVSQYH ELVVQARDDF RKELDSITPD 300
ITPGWKGMSI SDLAGKLSTD DLNSLIAHAH RRIDQLNREL AQQKATEKQH IELALEKHKL 360
EEKRTFDSAV AKALEHHRSE IQAEQDRKVE EVRDAMENEM RTQLRRQAAA HTDHLRDVLK 420
VQEQELKYEF EQGLSEKLSE QELEFRRRSQ EQMDSFTLDI NTAYARLRGI EQAVQSHAVA 480
EEEARKAHQL WLSVEALKYS MKTSSAEMPT IPLGSAVEAI RVNCSDNEFT QALTAAIPPE 540
SLTRGVYSEE TLRARFYAVQ KLARRVAMID ETRNSLYQYF LSYLQSLLLF PPKQLKPPAE 600
LYPEDINTFK LLSYASYCIE HGDLELAAKF VNQLKGESRR VAQDWLKEAR MTLETKQIVE 660
ILTAYASAVG IGTTQVQQE 679 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-KW.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0051560; P:mitochondrial calcium ion homeostasis; IMP:MGI. 
Interpro
 IPR019133; Mt-IM_prot_Mitofilin. 
Pfam
 PF09731; Mitofilin 
SMART
  
PROSITE
  
PRINTS