CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006381
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleolar pre-ribosomal-associated protein 1 
Protein Synonyms/Alias
 Unhealthy ribosome biogenesis protein 1 
Gene Name
 URB1 
Gene Synonyms/Alias
 NPA1; YKL014C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
22REKYTQGKEFEDGTLubiquitination[1]
594LLKLASSKNASNVITubiquitination[1]
1565PTAPLHEKAYRWVLSacetylation[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Required for 60S ribosomal subunit formation and pre- rRNA processing. Required for normal accumulation of 25S and 5.8S rRNAs. 
Sequence Annotation
  
Keyword
 Complete proteome; Nucleus; Reference proteome; Ribosome biogenesis; rRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1764 AA 
Protein Sequence
MSNHSEAYGS RDQRREKYTQ GKEFEDGTLE TLESIISAVE DETLSKDYQP LIVFFQRGFG 60
AQLVQTWSYY AQVNNHGKFS KTTSLLTKTL RVLSSDTSTV TIGSGLIRLI LTDYTKVLYR 120
GLNNMRAQLT NPILRLLKQI VNFNNGQHIE ELVSYFDFSL PILPRLLVPS KSELANGNSS 180
ADSSKHDSLR FTFIKFWLTL ISNASPFVRK ELLTENFKIM SNLFKFMNKA DSDKLSEHIL 240
SVFINDILKE KSFKRTTKTK ILNELAASKI HHFYYSSNKN LVKKANEFFL TFGASRDFSV 300
AFPDNCVWFK NSVADGASHG APITVNQVEF QIHNKLLFNT LRLFKPWEDT LQLGTLIKIL 360
ENVPELVAPY SIFLTTNGNY DPKMTSYWFG ITLLINKIIN LKIPQFMEKV DSNIPPATSL 420
VIENILPSLL TKSSLVKSLQ FETPIIRQLA CQSIVLALKK LEKVSTFYDQ KGWRNEKTIL 480
LNEFHTRIPD LPIFVSTLSN SLASNKDNRI LPLSISIIFN YYSKMFPNLF SINLPSSNIY 540
TDIMQKSKIS GIEFAILDNY LQFQEFNSTQ TRWWNPSSGG NSLFTLLLKL ASSKNASNVI 600
TTRISNLLDE LTRTNVIFNI SLISPVMALV NSLQGLSLQV SEIDNMEQVW KWFDETISRV 660
VKTPYKYVDM AKEYNYISPF IMCLSEQWKY VDKSGNPEFL IKWLILFLRN MIFIGEDHIG 720
IDKLVKNVFP EVSDHDVNIY LKLDSFEENI KKTNSSNSLI SSMKSSSFFQ YISALPSKNL 780
MNISRLPVNK LDAAGILFRV QLLVEDDSVV YDNWFEATAC ELTGKIASYM VTDTEFPIIK 840
VLERYINFAL PKLAIEKRNA LLMKKSRFMC NLIGAVCFET GHQLVEFREI IQKVVFSGEN 900
VEEYANYNEL YQKEDVNAFL TSVSEYLSTS ALTSLLMCST KLESTRNILQ KLFNEGKTIK 960
ISLVKNILNK AANEDPASIK EVNISLAKFF EENKVCVDAS SDPMGKLSLS ETTSLINSFV 1020
SSDLNYLVLK AFYRWEHFSF PSFIPSIWRI KDSPLLSIVT TAALFKHMQD KDFSAFAHET 1080
ISKYGNEIAK STYTTSKSEI FDEILNMITT YIDFYDETKR NEILKCVLSQ SDHKYHAATV 1140
RYIAAHNNFT YPGVETWLHK TLLYLTKYLS ERKVISNSFF ELLRAMAELL KLEEVPNKLN 1200
VKIINSQLEA ILGSEWIKQI KVLEYVIVLI FCVSKKSIQS QRMVQLLLSN DSYSSIMIKD 1260
NDEDSSYRKF LSTMILFSLF SIDPVVNSTP IVQEKLLTFY SGTISSNDKL ILKILETIES 1320
HTATSWTNMI FSWEFIKDEE EEILEAIGDT RLITKEREGL ILTLQKNMIK KSIDRYVLER 1380
PQVPELYTDS NTNNYDATTR CDLVKKYYDD TERSGVDMYD PLFLLLLIIH NKELVKMVKD 1440
DEGNVTYRYE FENFLDCKIF QFIICSLSDC HTVANISYEH LSNLASSLEK KTAQMNLEKQ 1500
ITSKDNERKE SDSDLIKYNS IYQVLIKRIL YQRQQNQDPI NPLIWFSISR IVDLLGSPTA 1560
PLHEKAYRWV LSNSTIRSWD IPMVSDVMMS YNKRQQDDNK KEIDMEIYYG ELSWVLTTIC 1620
KGIKTDEDYK MLEKKGVFEW LLNLINMPYL KERLRELIYF IFYKVQRVAD DGGLNLISRN 1680
GIVSFFEVLN NNIKSRLPQD DILNNIGTLR NENRGTLNTT LRLAQEQNGI EKLLLGYNEL 1740
VKSQKRLILW TEGDSDNVVK RLRK 1764 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:SGD.
 GO:0000466; P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
 GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD. 
Interpro
 IPR021714; Npa1_N. 
Pfam
 PF11707; Npa1 
SMART
  
PROSITE
  
PRINTS