CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-035955
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Threonine--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
  
Gene Name
 TARS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
5***MFEEKASSPSGKubiquitination[1]
12KASSPSGKMGGEEKPubiquitination[1]
18GKMGGEEKPIGAGEEacetylation[2]
18GKMGGEEKPIGAGEEubiquitination[1]
26PIGAGEEKQKEGGKKubiquitination[1]
65LEMYNILKAEHDSILubiquitination[1, 3, 4]
75HDSILAEKAEKDSKPubiquitination[1, 3, 4, 5]
91KVTLPDGKQVDAESWubiquitination[1, 3]
99QVDAESWKTTPYQIAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 129 AA 
Protein Sequence
MFEEKASSPS GKMGGEEKPI GAGEEKQKEG GKKKNKEGSG DGGRAELNPW PEYIYTRLEM 60
YNILKAEHDS ILAEKAEKDS KPIKVTLPDG KQVDAESWKT TPYQIACGIS QGLADNTVIA 120
KVNNVVWDL 129 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IDA:HPA.
 GO:0005737; C:cytoplasm; IDA:HPA. 
Interpro
 IPR012675; Beta-grasp_dom.
 IPR004095; TGS.
 IPR012676; TGS-like. 
Pfam
 PF02824; TGS 
SMART
  
PROSITE
  
PRINTS