CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005941
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 3-methyl-2-oxobutanoate hydroxymethyltransferase 
Protein Synonyms/Alias
 Ketopantoate hydroxymethyltransferase; KPHMT 
Gene Name
 panB 
Gene Synonyms/Alias
 b0134; JW0130 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
2******MKPTTISLLacetylation[1]
11TTISLLQKYKQEKKRacetylation[1]
151VNIFGGYKVQGRGDEacetylation[1]
228ITGGHIPKFAKNFLAacetylation[1]
231GHIPKFAKNFLAETGacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate. 
Sequence Annotation
 REGION 45 46 Alpha-ketoisovalerate binding.
 ACT_SITE 181 181 Proton acceptor.
 METAL 45 45 Magnesium.
 METAL 84 84 Magnesium.
 METAL 114 114 Magnesium (By similarity).
 BINDING 84 84 Alpha-ketoisovalerate.
 BINDING 112 112 Alpha-ketoisovalerate.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Magnesium; Metal-binding; Pantothenate biosynthesis; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 264 AA 
Protein Sequence
MKPTTISLLQ KYKQEKKRFA TITAYDYSFA KLFADEGLNV MLVGDSLGMT VQGHDSTLPV 60
TVADIAYHTA AVRRGAPNCL LLADLPFMAY ATPEQAFENA ATVMRAGANM VKIEGGEWLV 120
ETVQMLTERA VPVCGHLGLT PQSVNIFGGY KVQGRGDEAG DQLLSDALAL EAAGAQLLVL 180
ECVPVELAKR ITEALAIPVI GIGAGNVTDG QILVMHDAFG ITGGHIPKFA KNFLAETGDI 240
RAAVRQYMAE VESGVYPGEE HSFH 264 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0003864; F:3-methyl-2-oxobutanoate hydroxymethyltransferase activity; IDA:EcoCyc.
 GO:0000287; F:magnesium ion binding; IDA:EcoCyc.
 GO:0033317; P:pantothenate biosynthetic process from valine; IMP:EcoCyc. 
Interpro
 IPR003700; Pantoate_hydroxy_MeTrfase.
 IPR015813; Pyrv/PenolPyrv_Kinase-like_dom. 
Pfam
 PF02548; Pantoate_transf 
SMART
  
PROSITE
  
PRINTS