CPLM-019927

Genbank Nucleotide ID

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial

Protein Synonyms/Alias

Complex I-42kD; CI-42kD; NADH-ubiquinone oxidoreductase 42 kDa subunit

Mus musculus (Mouse)

Position	Peptide	Type	References
75	SGKNKLAKEIAQQLG	acetylation	[1, 2, 3]
122	EKFYDDPKSNDGNSY	acetylation	[1, 3, 4, 5, 6, 7, 8]
122	EKFYDDPKSNDGNSY	succinylation	[7]
122	EKFYDDPKSNDGNSY	ubiquitination	[9]
181	YNQGYIRKQCVDHYN	acetylation	[4]
191	VDHYNEIKRLTLPEY	acetylation	[3, 6, 8]
228	KGDPHEMKVTSAYLQ	acetylation	[3]
242	QDIENAYKKTFLPKM	acetylation	[3, 4, 6, 8]
277	VEDIEYLKYNKGPWL	acetylation	[6, 8]
277	VEDIEYLKYNKGPWL	ubiquitination	[9]
285	YNKGPWLKQDDWTFH	acetylation	[7]
285	YNKGPWLKQDDWTFH	succinylation	[7]
350	YNAEVGDKWIWLK**	acetylation	[1, 4, 6, 7]
350	YNAEVGDKWIWLK**	succinylation	[7]

[1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[4] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
[5] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
[6] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[7] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[8] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[9] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]

Functional Description

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

MOD_RES 122 122 N6-acetyllysine.
MOD_RES 181 181 N6-acetyllysine.
MOD_RES 242 242 N6-acetyllysine.
MOD_RES 350 350 N6-acetyllysine.

Acetylation; Complete proteome; Direct protein sequencing; Electron transport; FAD; Flavoprotein; Mitochondrion; Reference proteome; Respiratory chain; Transit peptide; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
GO:0005747; C:mitochondrial respiratory chain complex I; IEA:Compara.
GO:0005739; C:mitochondrion; IDA:MGI.
GO:0005524; F:ATP binding; IEA:InterPro.
GO:0016773; F:phosphotransferase activity, alcohol group as acceptor; IEA:InterPro.
GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
GO:0006139; P:nucleobase-containing compound metabolic process; IEA:InterPro.
GO:0042493; P:response to drug; IEA:Compara.

IPR002624; Deoxynucleoside_kinase.
IPR015828; NADH_UbQ_OxRdtase_42KD_su.
IPR027417; P-loop_NTPase.