CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024479
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Metalloprotease TIKI2 
Protein Synonyms/Alias
 Heart, kidney and adipose-enriched transmembrane protein homolog; TRAB domain-containing protein 2B 
Gene Name
 TRABD2B 
Gene Synonyms/Alias
 HKAT; TIKI2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
143MTPAQRGKGLYADYLubiquitination[1, 2]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Metalloprotease that acts as a negative regulator of the Wnt signaling pathway by mediating the cleavage of the 8 N- terminal residues of a subset of Wnt proteins. Following cleavage, Wnt proteins become oxidized and form large disulfide-bond oligomers, leading to their inactivation. Able to cleave WNT3A, WNT5, but not WNT11. Required for head formation. 
Sequence Annotation
 CARBOHYD 228 228 N-linked (GlcNAc...) (Potential).
 CARBOHYD 335 335 N-linked (GlcNAc...) (Potential).  
Keyword
 Cell membrane; Complete proteome; Glycoprotein; Hydrolase; Membrane; Metal-binding; Metalloprotease; Protease; Reference proteome; Signal; Transmembrane; Transmembrane helix; Wnt signaling pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 517 AA 
Protein Sequence
MHAALAGPLL AALLATARAR PQPPDGGQCR PPGSQRDLNS FLWTIRRDPP AYLFGTIHVP 60
YTRVWDFIPD NSKAAFQAST RVYFELDLTD PYTISALASC QLLPHGENLQ DVLPHELYWR 120
LKRHLDYVKL MMPSWMTPAQ RGKGLYADYL FNAIAGNWER KRPVWVMLMV NSLTERDVRF 180
RGVPVLDLYL AQQAEKMKKT TGAVEQVEEQ CHPLNNGLNF SQVLFALNQT LLQQESVRAG 240
SLQASYTTED LIKHYNCGDL SAVIFNHDTS QLPNFINTTL PPHEQVTAQE IDSYFRQELI 300
YKRNERMGKR VMALLRENED KICFFAFGAG HFLGNNTVID ILRQAGLEVD HTPAGQAIHS 360
PAPQSPAPSP EGTSTSPAPV TPAAAVPEAP SVTPTAPPED EDPALSPHLL LPDSLSQLEE 420
FGRQRKWHKR QSTHQRPRQF NDLWVRIEDS TTASPPPLPL QPTHSSGTAK PPFQLSDQLQ 480
QQDPPGPASS SAPTLGLLPA IATTIAVCFL LHSLGPS 517 
Gene Ontology
 GO:0031301; C:integral to organelle membrane; IDA:UniProtKB.
 GO:0005887; C:integral to plasma membrane; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004222; F:metalloendopeptidase activity; IDA:UniProtKB.
 GO:0017147; F:Wnt-protein binding; IDA:UniProtKB.
 GO:0030178; P:negative regulation of Wnt receptor signaling pathway; IDA:UniProtKB.
 GO:0018158; P:protein oxidation; IDA:UniProtKB.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 
Interpro
 IPR002816; Pheromone_shutdown_TraB. 
Pfam
 PF01963; TraB 
SMART
  
PROSITE
  
PRINTS