CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010205
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Beta-hexosaminidase 
Protein Synonyms/Alias
 Beta-N-acetylhexosaminidase; N-acetyl-beta-glucosaminidase 
Gene Name
 nagZ 
Gene Synonyms/Alias
 ycfO; b1107; JW1093 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
141SYHADPQKALAIASRacetylation[1]
177AVTADSHKETPCDPRacetylation[1]
232SGSPYWLKTVLRQELacetylation[1]
283ILVCNNRKGAVSVLDpupylation[2]
296LDNLSPIKAERVTRLacetylation[1]
338HERWQEEKAGH****acetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222] 
Functional Description
 Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides. 
Sequence Annotation
 ACT_SITE 248 248 By similarity.  
Keyword
 Cell cycle; Cell division; Cell shape; Cell wall biogenesis/degradation; Complete proteome; Cytoplasm; Glycosidase; Hydrolase; Peptidoglycan synthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 341 AA 
Protein Sequence
MGPVMLDVEG YELDAEEREI LAHPLVGGLI LFTRNYHDPA QLRELVRQIR AASRNRLVVA 60
VDQEGGRVQR FREGFTRLPA AQSFAALSGM EEGGKLAQEA GWLMASEMIA MDIDISFAPV 120
LDVGHISAAI GERSYHADPQ KALAIASRFI DGMHEAGMKT TGKHFPGHGA VTADSHKETP 180
CDPRPQAEIR AKDMSVFSSL IRENKLDAIM PAHVIYSDVD PRPASGSPYW LKTVLRQELG 240
FDGVIFSDDL SMEGAAIMGS YAERGQASLD AGCDMILVCN NRKGAVSVLD NLSPIKAERV 300
TRLYHKGSFS RQELMDSARW KAISTRLNQL HERWQEEKAG H 341 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004563; F:beta-N-acetylhexosaminidase activity; IEA:HAMAP.
 GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-KW.
 GO:0009254; P:peptidoglycan turnover; IMP:EcoliWiki.
 GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW. 
Interpro
 IPR022956; Beta_hexosaminidase_bac.
 IPR019800; Glyco_hydro_3_AS.
 IPR001764; Glyco_hydro_3_N.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF00933; Glyco_hydro_3 
SMART
  
PROSITE
 PS00775; GLYCOSYL_HYDROL_F3 
PRINTS