CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044021
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Mitofusin-1 
Protein Synonyms/Alias
  
Gene Name
 MFN1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
149RIFFVSAKEVLSARKubiquitination[1, 2]
199SQSAVKTKFEQHTIRubiquitination[1]
208EQHTIRAKQILATVKubiquitination[1, 2]
228VNLAAEDKRHYSVEEubiquitination[1, 2, 3]
258NLLTLDVKKKIKEVTubiquitination[1, 2, 3, 4, 5]
259LLTLDVKKKIKEVTEubiquitination[1, 2]
260LTLDVKKKIKEVTEEubiquitination[1]
262LDVKKKIKEVTEEVAubiquitination[2, 6]
302HPNPDVLKIYKSLPRubiquitination[1, 3, 6, 7]
395HAKERAFKQQFVNYAubiquitination[6, 7]
405FVNYATEKLRMIVSSubiquitination[1, 2, 3, 7]
422ANCSHQVKQQIATTFubiquitination[7]
441QQVDITQKQLEEEIAubiquitination[1, 6, 7]
459KEIDQLEKIQNNSKLubiquitination[1, 2]
465EKIQNNSKLLRNKAVubiquitination[1, 2]
470NSKLLRNKAVQLENEubiquitination[1, 2, 3, 6]
483NELENFTKQFLPSSNubiquitination[2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 493 AA 
Protein Sequence
TVNQLAHALH MDKDLKAGCL VRVFWPKAKC ALLRDDLVLV DSPGTDVTTE LDSWIDKFCL 60
DADVFVLVAN SESTLMNTEK HFFHKVNERL SKPNIFILNN RWDASASEPE YMEDVRRQHM 120
ERCLHFLVEE LKVVNALEAQ NRIFFVSAKE VLSARKQKAQ GMPESGVALA EGFHARLQEF 180
QNFEQIFEEC ISQSAVKTKF EQHTIRAKQI LATVKNIMDS VNLAAEDKRH YSVEEREDQI 240
DRLDFIRNQM NLLTLDVKKK IKEVTEEVAN KVSCAMTDEI CRLSVLVDEF CSEFHPNPDV 300
LKIYKSLPRS LASTPTAPTT PATPDNASQE ELMITLVTGL ASVTSRTSMG IIIVGGVIWK 360
TIGWKLLSVS LTMYGALYLY ERLSWTTHAK ERAFKQQFVN YATEKLRMIV SSTSANCSHQ 420
VKQQIATTFA RLCQQVDITQ KQLEEEIARL PKEIDQLEKI QNNSKLLRNK AVQLENELEN 480
FTKQFLPSSN EES 493 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:InterPro.
 GO:0005741; C:mitochondrial outer membrane; IEA:InterPro.
 GO:0005525; F:GTP binding; IEA:InterPro.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0008053; P:mitochondrial fusion; IEA:InterPro. 
Interpro
 IPR001401; Dynamin_GTPase.
 IPR006884; Fzo/mitofusin_HR2.
 IPR027088; Mitofusin-1.
 IPR027094; Mitofusin_fam.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00350; Dynamin_N
 PF04799; Fzo_mitofusin 
SMART
  
PROSITE
  
PRINTS