UniProt Accession

 CYH2_HUMAN; Q99418; A8K8P0; Q8IXY9; Q92958 

Genbank Protein ID

 X99753; U70728; AK292405; BC004361; BC038713 

Genbank Nucleotide ID

 CAA68084.1; AAB09591.1; BAF85094.1; AAH04361.1; AAH38713.1 

Protein Name

 Cytohesin-2 

Protein Synonyms/Alias

 ARF exchange factor; ARF nucleotide-binding site opener; Protein ARNO; PH, SEC7 and coiled-coil domain-containing protein 2 

Gene Name

 CYTH2 

Gene Synonyms/Alias

 ARNO; PSCD2; PSCD2L 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
26	LENIRRRKQELLVEI	ubiquitination	[1]
57	LEANEGSKTLQRNRK	ubiquitination	[1]
70	RKMAMGRKKFNMDPK	ubiquitination	[1]
71	KMAMGRKKFNMDPKK	ubiquitination	[1]
78	KFNMDPKKGIQFLVE	ubiquitination	[1]
102	EIARFLYKGEGLNKT	ubiquitination	[2]
159	RLPGEAQKIDRMMEA	ubiquitination	[1]
319	REVDDPRKPNCFELY	ubiquitination	[1]
364	SAPTQEEKDEWIKSI	ubiquitination	[1]
369	EEKDEWIKSIQAAVS	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. 

Sequence Annotation

 DOMAIN 72 201 SEC7.
 DOMAIN 259 376 PH.  

Keyword

 3D-structure; Alternative splicing; Cell membrane; Coiled coil; Complete proteome; Cytoplasm; Guanine-nucleotide releasing factor; Membrane; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 400 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0005086; F:ARF guanyl-nucleotide exchange factor activity; IBA:RefGenome.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0030036; P:actin cytoskeleton organization; TAS:ProtInc.
 GO:0006897; P:endocytosis; TAS:ProtInc.
 GO:0032012; P:regulation of ARF protein signal transduction; IEA:InterPro.
 GO:0030155; P:regulation of cell adhesion; IBA:RefGenome. 

Interpro

 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR000904; Sec7.
 IPR023394; SEC7_alpha_orthog. 

Pfam

 PF00169; PH
 PF01369; Sec7 

SMART

 SM00233; PH
 SM00222; Sec7 

PROSITE

 PS50003; PH_DOMAIN
 PS50190; SEC7 

PRINTS