UniProt Accession

 PIAS2_HUMAN; O75928; O75927; Q96BT5; Q96KE3 

Genbank Protein ID

 AF077953; AF077954; AF361054; AC090241; AC090373; BC015190 

Genbank Nucleotide ID

 AAC36704.1; AAC36705.1; AAK48938.1; AAH15190.1 

Protein Name

 E3 SUMO-protein ligase PIAS2 

Protein Synonyms/Alias

 Androgen receptor-interacting protein 3; ARIP3; DAB2-interacting protein; DIP; Msx-interacting zinc finger protein; Miz1; PIAS-NY protein; Protein inhibitor of activated STAT x; Protein inhibitor of activated STAT2 

Gene Name

 PIAS2 

Gene Synonyms/Alias

 PIASX 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
46	MRALHLLKSGCSPAV	ubiquitination	[1]
249	PGYAPPPKNGIEQKR	ubiquitination	[1, 2]
326	SRALIKEKLTADPDS	ubiquitination	[3, 4]
443	VSSQPCTKIESSSVL	ubiquitination	[1]
452	ESSSVLSKPCSVTVA	ubiquitination	[1]
464	TVASEASKKKVDVID	ubiquitination	[1]
465	VASEASKKKVDVIDL	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2- alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys- 160'. 

Sequence Annotation

 DOMAIN 11 45 SAP.
 DOMAIN 134 299 PINIT.
 ZN_FING 331 408 SP-RING-type.
 REGION 467 473 SUMO1-binding.
 MOTIF 19 23 LXXLL motif.
 MOTIF 484 492 Nuclear localization signal (By
 MOD_RES 476 476 Phosphoserine.
 MOD_RES 477 477 Phosphoserine.
 MOD_RES 478 478 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; DNA-binding; Ligase; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 621 AA 

Protein Sequence

Gene Ontology

 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
 GO:0050681; F:androgen receptor binding; NAS:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0019789; F:SUMO ligase activity; IDA:BHF-UCL.
 GO:0003713; F:transcription coactivator activity; NAS:UniProtKB.
 GO:0008270; F:zinc ion binding; TAS:ProtInc.
 GO:0030521; P:androgen receptor signaling pathway; NAS:UniProtKB.
 GO:0060766; P:negative regulation of androgen receptor signaling pathway; IDA:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; NAS:UniProtKB.
 GO:0016925; P:protein sumoylation; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR027228; PIAS2.
 IPR023321; PINIT.
 IPR003034; SAP_dom.
 IPR004181; Znf_MIZ. 

Pfam

 PF14324; PINIT
 PF02891; zf-MIZ 

SMART

 SM00513; SAP 

PROSITE

 PS51466; PINIT
 PS50800; SAP
 PS51044; ZF_SP_RING 

PRINTS