UniProt Accession

 RBFA_ECOLI; P0A7G2; P09170; Q2M943 

Genbank Protein ID

 X13270; X13775; U18997; U00096; AP009048 

Genbank Nucleotide ID

 CAA31634.1; CAA32020.1; AAA57970.1; AAC76201.1; BAE77213.1 

Protein Name

 Ribosome-binding factor A 

Protein Synonyms/Alias

 Protein P15B 

Gene Name

 rbfA 

Gene Synonyms/Alias

 P15B; yhbB; b3167; JW3136 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
17	RVAQEMQKEIALILQ	acetylation	[1]
60	YVTFLNDKDEDAVKA	acetylation	[1]
66	DKDEDAVKAGIKALQ	acetylation	[1]
70	DAVKAGIKALQEASG	acetylation	[1]
85	FIRSLLGKAMRLRIV	acetylation	[1]
118	NLVTSVVKHDEERRV	acetylation	[1]
131	RVNPDDSKED*****	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Essential for efficient processing of 16S rRNA. Probably part of the 30S subunit prior to or during the final step in the processing of 16S free 30S ribosomal subunits. Could be some accessory protein needed for efficient assembly of the 30S subunit. May interact with the 5'-terminal helix region of 16S rRNA. Has affinity for free ribosomal 30S subunits but not for 70S ribosomes. Overexpression suppresses a cold-sensitive C23U 16S rRNA mutation and rimM deletion mutants. Its function probably overlaps Era. 

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Reference proteome; Ribosome biogenesis; rRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 133 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0030490; P:maturation of SSU-rRNA; IMP:EcoliWiki. 

Interpro

 IPR015946; KH_dom-like_a/b.
 IPR000238; Ribosome-bd_facA.
 IPR023799; Ribosome-bd_facA_dom.
 IPR020053; Ribosome-bd_factorA_CS. 

Pfam

 PF02033; RBFA 

SMART

  

PROSITE

 PS01319; RBFA 

PRINTS