CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004743
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ADP-ribosylation factor 2 
Protein Synonyms/Alias
  
Gene Name
 ARF2 
Gene Synonyms/Alias
 YDL137W; D2165 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
127VWLVFANKQDLPEAMubiquitination[1]
142SAAEITEKLGLHSIRubiquitination[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, VillĂ©n J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047
Functional Description
 GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. 
Sequence Annotation
 NP_BIND 25 32 GTP.
 NP_BIND 126 129 GTP.
 NP_BIND 160 161 GTP.
 BINDING 48 48 GTP (By similarity).
 BINDING 70 70 GTP; via amide nitrogen (By similarity).
 LIPID 2 2 N-myristoyl glycine (By similarity).  
Keyword
 3D-structure; Complete proteome; ER-Golgi transport; Golgi apparatus; GTP-binding; Lipoprotein; Myristate; Nucleotide-binding; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 181 AA 
Protein Sequence
MGLYASKLFS NLFGNKEMRI LMVGLDGAGK TTVLYKLKLG EVITTIPTIG FNVETVQYKN 60
ISFTVWDVGG QDRIRSLWRH YYRNTEGVIF VIDSNDRSRI GEAREVMQRM LNEDELRNAV 120
WLVFANKQDL PEAMSAAEIT EKLGLHSIRN RPWFIQSTCA TSGEGLYEGL EWLSNNLKNQ 180
S 181 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IDA:SGD.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IDA:SGD.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; TAS:SGD.
 GO:0006891; P:intra-Golgi vesicle-mediated transport; TAS:SGD.
 GO:0016236; P:macroautophagy; IMP:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR024156; Small_GTPase_ARF.
 IPR006689; Small_GTPase_ARF/SAR. 
Pfam
 PF00025; Arf 
SMART
 SM00177; ARF 
PROSITE
 PS51417; ARF 
PRINTS
 PR00328; SAR1GTPBP.