UniProt Accession

 VGFR1_MOUSE; P35969; O55094; Q61517 

Genbank Protein ID

 L07297; X78568; D88689 

Genbank Nucleotide ID

 AAA40078.1; CAA55311.1; BAA24498.1 

Protein Name

 Vascular endothelial growth factor receptor 1 

Protein Synonyms/Alias

 VEGFR-1; Embryonic receptor kinase 2; Fms-like tyrosine kinase 1; FLT-1; Tyrosine-protein kinase receptor FLT 

Gene Name

 Flt1 

Gene Synonyms/Alias

 Emrk2; Flt; Vegfr1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
792	KRSSSEVKTDYLSII	ubiquitination	[1]
820	RLPYDASKWEFARER	ubiquitination	[1]
841	LGRGAFGKVVQASAF	ubiquitination	[1]
851	QASAFGIKKSPTCRT	ubiquitination	[1]
865	TVAVKMLKEGATASE	ubiquitination	[1]
955	PGLEQGQKPRLDSVS	ubiquitination	[1]
1049	GLARDIYKNPDYVRR	ubiquitination	[1]
1153	RFAELVEKLGDLLQA	ubiquitination	[1]
1218	VRYVNAFKFMSLERI	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, and proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts. Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to the activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC, YES1 and PLCG, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 and PTK2/FAK1 (By similarity). 

Sequence Annotation

 DOMAIN 32 124 Ig-like C2-type 1.
 DOMAIN 152 215 Ig-like C2-type 2.
 DOMAIN 231 328 Ig-like C2-type 3.
 DOMAIN 334 429 Ig-like C2-type 4.
 DOMAIN 430 550 Ig-like C2-type 5.
 DOMAIN 557 656 Ig-like C2-type 6.
 DOMAIN 662 748 Ig-like C2-type 7.
 DOMAIN 828 1158 Protein kinase.
 NP_BIND 834 842 ATP (By similarity).
 ACT_SITE 1022 1022 Proton acceptor (By similarity).
 BINDING 862 862 ATP (By similarity).
 MOD_RES 915 915 Phosphotyrosine; by autocatalysis (By
 MOD_RES 1053 1053 Phosphotyrosine; by autocatalysis (By
 MOD_RES 1169 1169 Phosphotyrosine; by autocatalysis (By
 MOD_RES 1213 1213 Phosphotyrosine; by autocatalysis (By
 MOD_RES 1242 1242 Phosphotyrosine; by autocatalysis (By
 MOD_RES 1263 1263 Phosphothreonine.
 MOD_RES 1322 1322 Phosphotyrosine; by autocatalysis (By
 MOD_RES 1328 1328 Phosphotyrosine; by autocatalysis (By
 CARBOHYD 101 101 N-linked (GlcNAc...) (Potential).
 CARBOHYD 165 165 N-linked (GlcNAc...) (Potential).
 CARBOHYD 197 197 N-linked (GlcNAc...) (Potential).
 CARBOHYD 252 252 N-linked (GlcNAc...) (Potential).
 CARBOHYD 324 324 N-linked (GlcNAc...) (Potential).
 CARBOHYD 418 418 N-linked (GlcNAc...) (Potential).
 CARBOHYD 475 475 N-linked (GlcNAc...) (Potential).
 CARBOHYD 517 517 N-linked (GlcNAc...) (Potential).
 CARBOHYD 598 598 N-linked (GlcNAc...) (Potential).
 CARBOHYD 626 626 N-linked (GlcNAc...) (Potential).
 CARBOHYD 667 667 N-linked (GlcNAc...) (Potential).
 CARBOHYD 714 714 N-linked (GlcNAc...) (Potential).
 DISULFID 53 108 By similarity.
 DISULFID 159 208 By similarity.
 DISULFID 253 312 By similarity.
 DISULFID 455 536 By similarity.
 DISULFID 578 637 By similarity.
 DISULFID 683 732 By similarity.  

Keyword

 Angiogenesis; ATP-binding; Cell membrane; Chemotaxis; Complete proteome; Developmental protein; Differentiation; Direct protein sequencing; Disulfide bond; Endosome; Glycoprotein; Immunoglobulin domain; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1333 AA 

Protein Sequence

Gene Ontology

 GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IEA:Compara.
 GO:0005887; C:integral to plasma membrane; ISS:UniProtKB.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0036332; F:placental growth factor-activated receptor activity; IEA:Compara.
 GO:0005021; F:vascular endothelial growth factor-activated receptor activity; IDA:MGI.
 GO:0036326; F:VEGF-A-activated receptor activity; IEA:Compara.
 GO:0036327; F:VEGF-B-activated receptor activity; IEA:Compara.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0016477; P:cell migration; IMP:UniProtKB.
 GO:0048598; P:embryonic morphogenesis; IMP:UniProtKB.
 GO:0002548; P:monocyte chemotaxis; IEA:Compara.
 GO:0001569; P:patterning of blood vessels; IMP:MGI.
 GO:0018108; P:peptidyl-tyrosine phosphorylation; ISS:UniProtKB.
 GO:0045766; P:positive regulation of angiogenesis; IEA:Compara.
 GO:0030335; P:positive regulation of cell migration; IEA:Compara.
 GO:0043406; P:positive regulation of MAP kinase activity; IEA:Compara.
 GO:0043552; P:positive regulation of phosphatidylinositol 3-kinase activity; IEA:Compara.
 GO:0014068; P:positive regulation of phosphatidylinositol 3-kinase cascade; IEA:Compara.
 GO:0010863; P:positive regulation of phospholipase C activity; IEA:Compara.
 GO:0030949; P:positive regulation of vascular endothelial growth factor receptor signaling pathway; IEA:Compara.
 GO:0046777; P:protein autophosphorylation; ISS:UniProtKB.
 GO:0002040; P:sprouting angiogenesis; IMP:MGI.
 GO:0048010; P:vascular endothelial growth factor receptor signaling pathway; IMP:MGI. 

Interpro

 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR013098; Ig_I-set.
 IPR003599; Ig_sub.
 IPR003598; Ig_sub2.
 IPR013106; Ig_V-set.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR008266; Tyr_kinase_AS.
 IPR020635; Tyr_kinase_cat_dom.
 IPR001824; Tyr_kinase_rcpt_3_CS.
 IPR009135; Tyr_kinase_VEGFR1_rcpt_N.
 IPR009134; Tyr_kinase_VEGFR_rcpt_N. 

Pfam

 PF07679; I-set
 PF07714; Pkinase_Tyr
 PF07686; V-set 

SMART

 SM00409; IG
 SM00408; IGc2
 SM00219; TyrKc 

PROSITE

 PS50835; IG_LIKE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00109; PROTEIN_KINASE_TYR
 PS00240; RECEPTOR_TYR_KIN_III 

PRINTS

 PR01832; VEGFRECEPTOR.
 PR01833; VEGFRECEPTR1.