UniProt Accession

 RU2A_HUMAN; P09661; B2R5I6; Q8TBD2 

Genbank Protein ID

 X13482; AK312200; CH471101; BC022816; BC071717 

Genbank Nucleotide ID

 CAA31838.1; BAG35133.1; EAX02300.1; AAH22816.1; AAH71717.1 

Protein Name

 U2 small nuclear ribonucleoprotein A' 

Protein Synonyms/Alias

 U2 snRNP A' 

Gene Name

 SNRPA1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
138	YRLYVIYKVPQVRVL	ubiquitination	[1]
151	VLDFQKVKLKERQEA	ubiquitination	[1]
172	KRGAQLAKDIARRSK	acetylation	[2]
179	KDIARRSKTFNPGAG	ubiquitination	[3, 4, 5, 6]
191	GAGLPTDKKKGGPSP	ubiquitination	[4]
192	AGLPTDKKKGGPSPG	ubiquitination	[4]
205	PGDVEAIKNAIANAS	ubiquitination	[3, 4, 5, 6]
221	LAEVERLKGLLQSGQ	ubiquitination	[1, 3, 4, 5, 6]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 This protein is associated with sn-RNP U2. It helps the A' protein to bind stem loop IV of U2 snRNA. 

Sequence Annotation

 REPEAT 20 41 LRR 1.
 REPEAT 43 64 LRR 2.
 REPEAT 65 86 LRR 3.
 REPEAT 89 110 LRR 4.
 DOMAIN 123 161 LRRCT.
 MOD_RES 172 172 N6-acetyllysine.
 MOD_RES 178 178 Phosphoserine.
 MOD_RES 197 197 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Leucine-rich repeat; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Ribonucleoprotein; RNA-binding; Spliceosome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 255 AA 

Protein Sequence

Gene Ontology

 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005686; C:U2 snRNP; TAS:ProtInc.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0000398; P:mRNA splicing, via spliceosome; IC:UniProtKB. 

Interpro

 IPR001611; Leu-rich_rpt.
 IPR003603; U2A'_phosphoprotein32A_C. 

Pfam

  

SMART

 SM00446; LRRcap 

PROSITE

 PS51450; LRR 

PRINTS