CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-040017
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Phenylalanine--tRNA ligase beta subunit 
Protein Synonyms/Alias
  
Gene Name
 FARSB 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MPDGKIQKLIITEETacetylation[1, 2]
8MPDGKIQKLIITEETubiquitination[3]
46SFIELQEKLHQNICRubiquitination[3]
230ETPENLAKLLTRMYLubiquitination[3, 4]
296ANQFPLNKLTELLRHubiquitination[3]
344AVHISNPKTAEFQVAubiquitination[2, 3]
361TLLPGLLKTIAANRKubiquitination[2, 3, 5]
393SNTDVGAKNYRHLCAubiquitination[3]
461ARGQSVGKLGVLHPDubiquitination[2, 3, 4, 5, 6]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 490 AA 
Protein Sequence
MPDGKIQKLI ITEETAKIRP FAVAAVLRNI KFTKDRYDSF IELQEKLHQN ICRKRALVAI 60
GTHDLDTLSG PFTYTAKRPS DIKFKPLNKT KEYTACELMN IYKTDNHLKH YLHIIENKPL 120
YPVIYDSNGV VLSMPPIING DHSRITVNTR NIFIECTGTD FTKAKIVLDI IVTMFSEYCE 180
NQFTVEAAEV VFPNGKSHTF PELAYRKEMV RADLINKKVG IRETPENLAK LLTRMYLKSE 240
VIGDGNQIEI EIPPTRADII HACDIVEDAA IAYGYNNIQM TLPKTYTIAN QFPLNKLTEL 300
LRHDMAAAGF TEALTFALCS QEDIADKLGV DISATKAVHI SNPKTAEFQV ARTTLLPGLL 360
KTIAANRKMP LPLKLFEISD IVIKDSNTDV GAKNYRHLCA VYYNKNPGFE IIHGLLDRIM 420
QLLDVPPGED KGGYVIKASE GPAFFPGRCA EIFARGQSVG KLGVLHPDVI TKFELTMPCS 480
SLEINVGPFL 490 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0004826; F:phenylalanine-tRNA ligase activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0006432; P:phenylalanyl-tRNA aminoacylation; IEA:InterPro. 
Interpro
 IPR005146; B3/B4_tRNA-bd.
 IPR009061; DNA-bd_dom_put.
 IPR004531; Phe-tRNA-synth_IIc_bsu_arc.
 IPR020825; Phe-tRNA_synthase_B3/B4.
 IPR005147; tRNA_synthase_B5-dom. 
Pfam
 PF03483; B3_4
 PF03484; B5 
SMART
 SM00873; B3_4
 SM00874; B5 
PROSITE
 PS51483; B5 
PRINTS