CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009933
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha 
Protein Synonyms/Alias
 PIP5K1-alpha; PtdIns(4)P-5-kinase 1 alpha; 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase; Phosphatidylinositol 4-phosphate 5-kinase type I alpha; PIP5KIalpha; Phosphatidylinositol 4-phosphate 5-kinase type I beta; PI4P5KIbeta 
Gene Name
 Pip5k1a 
Gene Synonyms/Alias
 Pip5k1b 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
88TVGSLSTKPERDVLMubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113
Functional Description
 Catalyzes the phosphorylation of phosphatidylinositol 4- phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. 
Sequence Annotation
 DOMAIN 66 434 PIPK.
 CROSSLNK 88 88 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative splicing; ATP-binding; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Isopeptide bond; Kinase; Membrane; Nucleotide-binding; Nucleus; Reference proteome; Transferase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 546 AA 
Protein Sequence
MASASSGPAA AGFSSLDAGA PAGTAAASGI KRATVSEGPS ASVMPVKKIG HRSVDSSGET 60
TYKKTTSSAL KGAIQLGITH TVGSLSTKPE RDVLMQDFYV VESIFFPSEG SNLTPAHHYN 120
DFRFKTYAPV AFRYFRELFG IRPDDYLYSL CSEPLIELSN SGASGSLFYV SSDDEFIIKT 180
VQHKEAEFLQ KLLPGYYMNL NQNPRTLLPK FYGLYCVQAG GKNIRIVVMN NLLPRSVKMH 240
MKYDLKGSTY KRRASQKERE KTLPTFKDLD FLQDIPDGLF LDADMYSALC KTLQRDCLVL 300
QSFKIMDYSL LMSIHNMDHA QREPTSNDTQ YSADTRRPAP QKALYSTAME SIQGEARRGG 360
TVETEDHMGG IPARNNKGER LLLYIGIIDI LQSYRFVKKL EHSWKALVHD GDTVSVHRPG 420
FYAERFQRFM CNTVFKKIPL KPSPTKKFRS GPSFSRRSGP SGNSCTSQLM ASGEHRAQVT 480
TKAEVEPDVH LGRPDVLPQT PPLEEISEGS PVPGPSFSPV VGQPLQILNL SSTLEKLDVA 540
ESEFTH 546 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0030027; C:lamellipodium; IEA:Compara.
 GO:0005847; C:mRNA cleavage and polyadenylation specificity factor complex; IEA:Compara.
 GO:0016607; C:nuclear speck; ISS:UniProtKB.
 GO:0032587; C:ruffle membrane; IDA:MGI.
 GO:0016308; F:1-phosphatidylinositol-4-phosphate 5-kinase activity; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0031532; P:actin cytoskeleton reorganization; ISS:UniProtKB.
 GO:0032863; P:activation of Rac GTPase activity; ISS:UniProtKB.
 GO:0060326; P:cell chemotaxis; ISS:UniProtKB.
 GO:0010761; P:fibroblast migration; IMP:MGI.
 GO:0048041; P:focal adhesion assembly; ISS:UniProtKB.
 GO:0008654; P:phospholipid biosynthetic process; IEA:Compara.
 GO:0072661; P:protein targeting to plasma membrane; ISS:UniProtKB.
 GO:0097178; P:ruffle assembly; ISS:UniProtKB. 
Interpro
 IPR023610; PInositol-4-P-5-kinase.
 IPR027483; PInositol-4-P-5-kinase_C.
 IPR002498; PInositol-4-P-5-kinase_core.
 IPR027484; PInositol-4-P-5-kinase_N.
 IPR016034; PInositol-4P-5-kinase_core_sub. 
Pfam
 PF01504; PIP5K 
SMART
 SM00330; PIPKc 
PROSITE
 PS51455; PIPK 
PRINTS