CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022560
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kelch-like protein 9 
Protein Synonyms/Alias
  
Gene Name
 KLHL9 
Gene Synonyms/Alias
 KIAA1354 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
171YNLIEVDKYVNNFILubiquitination[1]
179YVNNFILKNFPALLSubiquitination[2]
192LSTGEFLKLPFERLAubiquitination[3]
235PRMDYAAKLMKNIRFubiquitination[3]
378RFDPRYNKWMQVASLubiquitination[1]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. 
Sequence Annotation
 DOMAIN 50 119 BTB.
 DOMAIN 154 255 BACK.
 REPEAT 300 347 Kelch 1.
 REPEAT 348 399 Kelch 2.
 REPEAT 400 446 Kelch 3.
 REPEAT 448 493 Kelch 4.
 REPEAT 495 545 Kelch 5.
 REPEAT 546 595 Kelch 6.  
Keyword
 Cell cycle; Cell division; Complete proteome; Kelch repeat; Mitosis; Reference proteome; Repeat; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 617 AA 
Protein Sequence
MKVSLGNGEM GVSAHLQPCK AGTTRFFTSN THSSVVLQGF DQLRIEGLLC DVTLVPGDGD 60
EIFPVHRAMM ASASDYFKAM FTGGMKEQDL MCIKLHGVNK VGLKKIIDFI YTAKLSLNMD 120
NLQDTLEAAS FLQILPVLDF CKVFLISGVS LDNCVEVGRI ANTYNLIEVD KYVNNFILKN 180
FPALLSTGEF LKLPFERLAF VLSSNSLKHC TELELFKAAC RWLRLEDPRM DYAAKLMKNI 240
RFPLMTPQDL INYVQTVDFM RTDNTCVNLL LEASNYQMMP YMQPVMQSDR TAIRSDSTHL 300
VTLGGVLRQQ LVVSKELRMY DERAQEWRSL APMDAPRYQH GIAVIGNFLY VVGGQSNYDT 360
KGKTAVDTVF RFDPRYNKWM QVASLNEKRT FFHLSALKGH LYAVGGRSAA GELATVECYN 420
PRMNEWSYVA KMSEPHYGHA GTVYGGLMYI SGGITHDTFQ NELMCFDPDT DKWMQKAPMT 480
TVRGLHCMCT VGDKLYVIGG NHFRGTSDYD DVLSCEYYSP TLDQWTPIAA MLRGQSDVGV 540
AVFENKIYVV GGYSWNNRCM VEIVQKYDPE KDEWHKVFDL PESLGGIRAC TLTVFPPEEN 600
PGSPSRESPL SAPSDHS 617 
Gene Ontology
 GO:0031463; C:Cul3-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0030496; C:midbody; IDA:UniProtKB.
 GO:0000910; P:cytokinesis; IMP:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0016567; P:protein ubiquitination; IDA:UniProtKB. 
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR015916; Gal_Oxidase_b-propeller.
 IPR017096; Kelch-like_gigaxonin.
 IPR006652; Kelch_1. 
Pfam
 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 
SMART
 SM00875; BACK
 SM00225; BTB
 SM00612; Kelch 
PROSITE
 PS50097; BTB 
PRINTS