CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021175
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA-directed RNA polymerase I subunit RPA49 
Protein Synonyms/Alias
 RNA polymerase I subunit A49; DNA-directed RNA polymerase I subunit E; RNA polymerase I-associated factor 1; RNA polymerase I-associated factor 53 
Gene Name
 POLR1E 
Gene Synonyms/Alias
 PAF53; PRAF1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
109RFTLYENKDSTNPRKubiquitination[1, 2]
142NFGTGALKCNTLCRHubiquitination[1, 2, 3, 4]
232SLNRAVAKAAETIIDubiquitination[5]
435KRMMEIAKAMRLKISacetylation[6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (By similarity). 
Sequence Annotation
 MOD_RES 97 97 Phosphoserine.
 MOD_RES 225 225 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; DNA-directed RNA polymerase; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 481 AA 
Protein Sequence
MYQASAVSLL PRDIPSCHSP SPGFSHLPTS SSQLAPDLLQ FPLGQDPSFL AIPILTLPPS 60
DSLVPPYIVW YIVWPSALIS FLGCTLTVQF SNGKLQSPGN MRFTLYENKD STNPRKRNQR 120
ILAAETDRLS YVGNNFGTGA LKCNTLCRHF VGILNKTSGQ MEVYDAELFN MQPLFSDVSV 180
ESELALESQT KTYREKMDSC IEAFGTTKQK RALNTRRMNR VGNESLNRAV AKAAETIIDT 240
KGVTALVSDA IHNDLQDDSL YLPPCYDDAA KPEDVYKFED LLSPAEYEAL QSPSEAFRNV 300
TSEEILKMIE ENSHCTFVIE ALKSLPSDVE SRDRQARCIW FLDTLIKFRA HRVVKRKSAL 360
GPGVPHIINT KLLKHFTCLT YNNGRLRNLI SDSMKAKITA YVIILALHIH DFQIDLTVLQ 420
RDLKLSEKRM MEIAKAMRLK ISKRRVSVAA GSEEDHKLGT LSLPLPPAQT SDRLAKRRKI 480
T 481 
Gene Ontology
 GO:0030054; C:cell junction; IDA:HPA.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005730; C:nucleolus; ISS:HGNC.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
 GO:0009303; P:rRNA transcription; ISS:HGNC. 
Interpro
 IPR009668; RNA_pol-assoc_fac_A49-like. 
Pfam
 PF06870; RNA_pol_I_A49 
SMART
  
PROSITE
  
PRINTS