CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019632
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Neurabin-2 
Protein Synonyms/Alias
 Neurabin-II; Protein phosphatase 1 regulatory subunit 9B; Spinophilin 
Gene Name
 PPP1R9B 
Gene Synonyms/Alias
 PPP1R6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
675KFKELQIKHAVTEAEubiquitination[1]
687EAEIQQLKRKLQSLEubiquitination[1]
689EIQQLKRKLQSLEQEubiquitination[1]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha- adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1 (By similarity). Required for hepatocyte growth factor (HGF)- induced cell migration. 
Sequence Annotation
 DOMAIN 494 582 PDZ.
 REGION 1 154 Actin-binding (By similarity).
 REGION 100 369 Interacts with D(2) dopamine receptor (By
 REGION 163 281 Actin-binding (By similarity).
 REGION 167 253 Interacts with ADRA2A, ADRA2B and ADRA2C
 REGION 415 492 Interacts with protein phosphatase 1 (By
 REGION 478 523 Interacts with RGS2 (By similarity).
 REGION 593 814 Interacts with TGN38 (By similarity).
 MOTIF 573 576 PP1-binding motif.
 MOD_RES 15 15 Phosphoserine (By similarity).
 MOD_RES 17 17 Phosphoserine (By similarity).
 MOD_RES 94 94 Phosphoserine (By similarity).
 MOD_RES 100 100 Phosphoserine (By similarity).
 MOD_RES 116 116 Phosphoserine (By similarity).
 MOD_RES 190 190 Phosphoserine.
 MOD_RES 203 203 Phosphoserine.  
Keyword
 Actin-binding; Cell junction; Cell membrane; Cell projection; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Developmental protein; Differentiation; Membrane; Neurogenesis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Synapse. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 815 AA 
Protein Sequence
MMKTEPRGPG GPLRSASPHR SAYEAGIQAL KPPDAPGPDE APKGAHHKKY GSNVHRIKSM 60
FLQMGTTAGP SGEAGGGAGL AEAPRASERG VRLSLPRASS LNENVDHSAL LKLGTSVSER 120
VSRFDSKPAP SAQPAPPPHP PSRLQETRKL FERSAPAAGG DKEAARRLLR QERAGLQDRK 180
LDVVVRFNGS TEALDKLDAD AVSPTVSQLS AVFEKADSRT GLHRGPGLPR AAGVPQVNSK 240
LVSKRSRVFQ PPPPPPPAPS GDAPAEKERC PAGQQPPQHR VAPARPPPKP REVRKIKPVE 300
VEESGESEAE SAPGEVIQAE VTVHAALENG STVATAASPA PEEPKAQAAP EKEAAAVAPP 360
ERGVGNGRAP DVAPEEVDES KKEDFSEADL VDVSAYSGLG EDSAGSALEE DDEDDEEDGE 420
PPYEPESGCV EIPGLSEEED PAPSRKIHFS TAPIQVFSTY SNEDYDRRNE DVDPMAASAE 480
YELEKRVERL ELFPVELEKD SEGLGISIIG MGAGADMGLE KLGIFVKTVT EGGAAHRDGR 540
IQVNDLLVEV DGTSLVGVTQ SFAASVLRNT KGRVRFMIGR ERPGEQSEVA QLIQQTLEQE 600
RWQREMMEQR YAQYGEDDEE TGEYATDEDE ELSPTFPGGE MAIEVFELAE NEDALSPVDM 660
EPEKLVHKFK ELQIKHAVTE AEIQQLKRKL QSLEQEKGRW RVEKAQLEQS VEENKERMEK 720
LEGYWGEAQS LCQAVDEHLR ETQAQYQALE RKYSKAKRLI KDYQQKEIEF LKKETAQRRV 780
LEESELARKE EMDKLLDKIS ELEGNLQTLR NSNST 815 
Gene Ontology
 GO:0005912; C:adherens junction; IEA:UniProtKB-SubCell.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0043197; C:dendritic spine; IEA:UniProtKB-SubCell.
 GO:0030175; C:filopodium; IDA:UniProtKB.
 GO:0030027; C:lamellipodium; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; IMP:UniProtKB.
 GO:0000164; C:protein phosphatase type 1 complex; TAS:UniProtKB.
 GO:0032587; C:ruffle membrane; IDA:UniProtKB.
 GO:0045202; C:synapse; IEA:UniProtKB-SubCell.
 GO:0008157; F:protein phosphatase 1 binding; NAS:UniProtKB.
 GO:0004864; F:protein phosphatase inhibitor activity; NAS:UniProtKB.
 GO:0007050; P:cell cycle arrest; TAS:UniProtKB.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0016477; P:cell migration; IMP:UniProtKB.
 GO:0071315; P:cellular response to morphine; ISS:UniProtKB.
 GO:0046847; P:filopodium assembly; IMP:UniProtKB.
 GO:0030308; P:negative regulation of cell growth; IDA:UniProtKB.
 GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
 GO:0001560; P:regulation of cell growth by extracellular stimulus; TAS:UniProtKB.
 GO:0042127; P:regulation of cell proliferation; NAS:UniProtKB.
 GO:0007096; P:regulation of exit from mitosis; NAS:UniProtKB.
 GO:2000474; P:regulation of opioid receptor signaling pathway; ISS:UniProtKB.
 GO:0008380; P:RNA splicing; NAS:UniProtKB. 
Interpro
 IPR001478; PDZ. 
Pfam
 PF00595; PDZ 
SMART
 SM00228; PDZ 
PROSITE
 PS50106; PDZ 
PRINTS