CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016098
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 D-beta-hydroxybutyrate dehydrogenase, mitochondrial 
Protein Synonyms/Alias
 BDH; 3-hydroxybutyrate dehydrogenase 
Gene Name
 Bdh1 
Gene Synonyms/Alias
 Bdh 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
89VFAGCLMKDKGDAGVacetylation[1]
91AGCLMKDKGDAGVKEacetylation[1]
97DKGDAGVKELDSLKSacetylation[1]
103VKELDSLKSDRLRTIacetylation[1]
122CNSEEVEKAVETIRSacetylation[1]
132ETIRSGLKDPEKGMWacetylation[1, 2]
232EMHPLGVKVSVVEPGacetylation[3]
258ERIQAIAKKMWDDLPacetylation[1, 3, 4]
259RIQAIAKKMWDDLPEacetylation[4, 5]
259RIQAIAKKMWDDLPEsuccinylation[5]
275VRKDYGRKYFDEKIAacetylation[2, 4]
280GRKYFDEKIAKMETYacetylation[1, 3, 4]
283YFDEKIAKMETYCNSacetylation[1]
339FPGAISDKIYIH***acetylation[1]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
  
Sequence Annotation
 NP_BIND 60 84 NAD (By similarity).
 ACT_SITE 209 209 Proton acceptor (By similarity).
 BINDING 196 196 Substrate (By similarity).
 MOD_RES 132 132 N6-acetyllysine.
 MOD_RES 275 275 N6-acetyllysine.  
Keyword
 Acetylation; Allosteric enzyme; Complete proteome; Direct protein sequencing; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 343 AA 
Protein Sequence
MLAARLSRPL SQLPGKALSV RDRENGTRHT LLFYPASFSP DTRRTYASQA DAASGKAILI 60
TGCDSGFGFS LAKHLHSKGF LVFAGCLMKD KGDAGVKELD SLKSDRLRTI QLNVCNSEEV 120
EKAVETIRSG LKDPEKGMWG LVNNAGISTF GEVEFTSMET YKEVAEVNLW GTVRTTKSFL 180
PLLRRAKGRV VNISSMLGRM ANPARSPYCI TKFGIEAFSD CLRYEMHPLG VKVSVVEPGN 240
FIAATSLYSP ERIQAIAKKM WDDLPEVVRK DYGRKYFDEK IAKMETYCNS GSTDTSSVIN 300
AVTHALTAAT PYTRYHPMDY YWWLRMQIMT HFPGAISDKI YIH 343 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0003858; F:3-hydroxybutyrate dehydrogenase activity; IEA:EC.
 GO:0005543; F:phospholipid binding; IEA:Compara.
 GO:0060612; P:adipose tissue development; IEA:Compara.
 GO:0007420; P:brain development; IEA:Compara.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0046686; P:response to cadmium ion; IEA:Compara.
 GO:0051412; P:response to corticosterone stimulus; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0032355; P:response to estradiol stimulus; IEA:Compara.
 GO:0045471; P:response to ethanol; IEA:Compara.
 GO:0060416; P:response to growth hormone stimulus; IEA:Compara.
 GO:0032868; P:response to insulin stimulus; IEA:Compara.
 GO:0007584; P:response to nutrient; IEA:Compara.
 GO:0042594; P:response to starvation; IEA:Compara.
 GO:0009636; P:response to toxic substance; IEA:Compara. 
Interpro
 IPR002198; DH_sc/Rdtase_SDR.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR020904; Sc_DH/Rdtase_CS. 
Pfam
 PF00106; adh_short 
SMART
  
PROSITE
 PS00061; ADH_SHORT 
PRINTS
 PR00081; GDHRDH.
 PR00080; SDRFAMILY.