UniProt Accession

 AL8A1_MOUSE; Q8BH00; Q91WS6 

Genbank Protein ID

 AF510322; AK050298; AK143752; BC013511; BC038493 

Genbank Nucleotide ID

 AAO64246.1; BAC34173.1; BAE25524.1; AAH13511.1; AAH38493.1 

Protein Name

 Aldehyde dehydrogenase family 8 member A1 

Protein Synonyms/Alias

 Retinal dehydrogenase 4 

Gene Name

 Aldh8a1 

Gene Synonyms/Alias

 Raldh4 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
38	STGEVYCKVPNSGKE	ubiquitination	[1]
44	CKVPNSGKEEIEAAV	ubiquitination	[1]
93	ELAQAESKDQGKTLT	ubiquitination	[1]
196	MFCKLLDKAGVPPGV	ubiquitination	[1]
248	QLSAPHCKKLSLELG	ubiquitination	[1]
249	LSAPHCKKLSLELGG	ubiquitination	[1]
305	SIYSEFLKRFVEATR	acetylation	[2, 3]
315	VEATRKWKVGVPSDP	ubiquitination	[1]
332	NMGALISKAHLEKVR	ubiquitination	[1]
344	KVRSYVLKAQTEGAR	acetylation	[4, 5]
344	KVRSYVLKAQTEGAR	ubiquitination	[1]
382	PTVITDIKDESRCMT	ubiquitination	[1]
426	LAATVWSKDVGRIHR	ubiquitination	[1]
461	NLPFGGMKSSGIGRE	ubiquitination	[1]
471	GIGREGAKDSYDFFT	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [5] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599] 

Functional Description

 Converts 9-cis-retinal to 9-cis-retinoic acid. Has lower activity towards 13-cis-retinal. Has much lower activity towards all-trans-retinal. Has a preference for NAD, but shows considerable activity with NADP (in vitro) (By similarity). 

Sequence Annotation

 NP_BIND 231 236 NAD (By similarity).
 ACT_SITE 253 253 Proton acceptor (By similarity).
 ACT_SITE 287 287 Nucleophile (By similarity).  

Keyword

 Complete proteome; Cytoplasm; NAD; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 487 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005622; C:intracellular; ISS:UniProtKB.
 GO:0001758; F:retinal dehydrogenase activity; ISS:UniProtKB.
 GO:0042904; P:9-cis-retinoic acid biosynthetic process; IDA:MGI.
 GO:0042574; P:retinal metabolic process; ISS:UniProtKB. 

Interpro

 IPR016161; Ald_DH/histidinol_DH.
 IPR016163; Ald_DH_C.
 IPR016160; Ald_DH_CS.
 IPR016162; Ald_DH_N.
 IPR015590; Aldehyde_DH_dom. 

Pfam

 PF00171; Aldedh 

SMART

  

PROSITE

 PS00070; ALDEHYDE_DEHYDR_CYS
 PS00687; ALDEHYDE_DEHYDR_GLU 

PRINTS