UniProt Accession

 RBP1_HUMAN; Q15311; D3DUI0 

Genbank Protein ID

 L42542; CH471113; CH471113; CH471113; CH471113; BC013126 

Genbank Nucleotide ID

 AAB00103.1; EAX01601.1; EAX01602.1; EAX01604.1; EAX01605.1; AAH13126.1 

Protein Name

 RalA-binding protein 1 

Protein Synonyms/Alias

 RalBP1; 76 kDa Ral-interacting protein; Dinitrophenyl S-glutathione ATPase; DNP-SG ATPase; Ral-interacting protein 1 

Gene Name

 RALBP1 

Gene Synonyms/Alias

 RLIP1; RLIP76 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
152	KHKEKKSKDLTAADV	ubiquitination	[1]
161	LTAADVVKQWKEKKK	ubiquitination	[2, 3, 4]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin. 

Sequence Annotation

 DOMAIN 192 380 Rho-GAP.
 REGION 403 499 Interacts with RalA.
 MOD_RES 2 2 N-acetylthreonine.
 MOD_RES 29 29 Phosphoserine (By similarity).
 MOD_RES 62 62 Phosphoserine.
 MOD_RES 92 92 Phosphoserine.
 MOD_RES 93 93 Phosphoserine.
 MOD_RES 463 463 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; GTPase activation; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 655 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0043492; F:ATPase activity, coupled to movement of substances; IDA:UniProtKB.
 GO:0030675; F:Rac GTPase activator activity; IDA:UniProtKB.
 GO:0006935; P:chemotaxis; TAS:ProtInc.
 GO:0043089; P:positive regulation of Cdc42 GTPase activity; IDA:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; IPI:UniProtKB.
 GO:0006810; P:transport; IDA:UniProtKB. 

Interpro

 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom. 

Pfam

 PF00620; RhoGAP 

SMART

 SM00324; RhoGAP 

PROSITE

 PS50238; RHOGAP 

PRINTS