UniProt Accession

 TOPK_HUMAN; Q96KB5; D3DST2; Q9NPD9; Q9NYL7; Q9NZK6 

Genbank Protein ID

 AB027249; AB027250; AF189722; AF237709; AK027291; CH471080; CH471080; BC015191 

Genbank Nucleotide ID

 BAA99576.1; BAA99577.1; AAF69107.1; AAF71521.1; BAB55019.1; EAW63536.1; EAW63537.1; AAH15191.1 

Protein Name

 Lymphokine-activated killer T-cell-originated protein kinase 

Protein Synonyms/Alias

 Cancer/testis antigen 84; CT84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinase 

Gene Name

 PBK 

Gene Synonyms/Alias

 TOPK 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
8	MEGISNFKTPSKLSE	ubiquitination	[1]
12	SNFKTPSKLSEKKKS	acetylation	[2]
64	SHSPWAVKKINPICN	acetylation	[3]
65	HSPWAVKKINPICND	ubiquitination	[1]
87	KRLMDEAKILKSLHH	ubiquitination	[1]
90	MDEAKILKSLHHPNI	ubiquitination	[1, 4]
132	DLIEERYKASQDPFP	ubiquitination	[1, 5]
155	LNMARGLKYLHQEKK	ubiquitination	[1]
169	KLLHGDIKSSNVVIK	ubiquitination	[1]
213	YIGTEPWKPKEAVEE	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. 

Sequence Annotation

 DOMAIN 32 322 Protein kinase.
 NP_BIND 38 46 ATP (By similarity).
 REGION 320 322 PDZ-interaction.
 ACT_SITE 167 167 Proton acceptor (By similarity).
 BINDING 64 64 ATP (By similarity).
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 9 9 Phosphothreonine.
 MOD_RES 24 24 Phosphothreonine.
 MOD_RES 32 32 Phosphoserine.
 MOD_RES 59 59 Phosphoserine.  

Keyword

 Acetylation; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 322 AA 

Protein Sequence

Gene Ontology

 GO:0005524; F:ATP binding; NAS:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; TAS:UniProtKB.
 GO:0007067; P:mitosis; NAS:UniProtKB. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

  

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS