CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023890
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RNA polymerase II subunit A C-terminal domain phosphatase 
Protein Synonyms/Alias
 TFIIF-associating CTD phosphatase 
Gene Name
 CTDP1 
Gene Synonyms/Alias
 FCP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
137DLTQLQSKNGKQQVPubiquitination[1]
283ECIDPFSKTGNLRNLubiquitination[1, 2]
319PNLITVKKYVYFQGTubiquitination[1]
614KYDRYLNKEIEEAPDubiquitination[1]
653PTNFPIEKTREHYHAubiquitination[1]
720SCLERWDKVEEQLFPubiquitination[1]
780IYDSNTGKLIRTGARacetylation[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. Plays a role in the exit from mitosis by dephosphorylating crucial mitotic substrates (USP44, CDC20 and WEE1) that are required for M-phase-promoting factor (MPF)/CDK1 inactivation. 
Sequence Annotation
 DOMAIN 178 344 FCP1 homology.
 DOMAIN 629 728 BRCT.
 MOD_RES 674 674 Phosphoserine.
 MOD_RES 780 780 N6-acetyllysine.
 MOD_RES 869 869 Phosphoserine.
 MOD_RES 872 872 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Cataract; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Hydrolase; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Protein phosphatase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 961 AA 
Protein Sequence
MEVPAAGRVP AEGAPTAAVA EVRCPGPAPL RLLEWRVAAG AAVRIGSVLA VFEAAASAQS 60
SGASQSRVAS GGCVRPARPE RRLRSERAGV VRELCAQPGQ VVAPGAVLVR LEGCSHPVVM 120
KGLCAECGQD LTQLQSKNGK QQVPLSTATV SMVHSVPELM VSSEQAEQLG REDQQRLHRN 180
RKLVLMVDLD QTLIHTTEQH CQQMSNKGIF HFQLGRGEPM LHTRLRPHCK DFLEKIAKLY 240
ELHVFTFGSR LYAHTIAGFL DPEKKLFSHR ILSRDECIDP FSKTGNLRNL FPCGDSMVCI 300
IDDREDVWKF APNLITVKKY VYFQGTGDMN APPGSRESQT RKKVNHSRGT EVSEPSPPVR 360
DPEGVTQAPG VEPSNGLEKP ARELNGSEAA TPRDSPRPGK PDERDIWPPA QAPTSSQELA 420
GAPEPQGSCA QGGRVAPGQR PAQGATGTDL DFDLSSDSES SSESEGTKSS SSASDGESEG 480
KRGRQKPKAA PEGAGALAQG SSLEPGRPAA PSLPGEAEPG AHAPDKEPEL GGQEEGERDG 540
LCGLGNGCAD RKEAETESQN SELSGVTAGE SLDQSMEEEE EEDTDEDDHL IYLEEILVRV 600
HTDYYAKYDR YLNKEIEEAP DIRKIVPELK SKVLADVAII FSGLHPTNFP IEKTREHYHA 660
TALGAKILTR LVLSPDAPDR ATHLIAARAG TEKVLQAQEC GHLHVVNPDW LWSCLERWDK 720
VEEQLFPLRD DHTKAQRENS PAAFPDREGV PPTALFHPMP VLPKAQPGPE VRIYDSNTGK 780
LIRTGARGPP APSSSLPIRQ EPSSFRAVPP PQPQMFGEEL PDAQDGEQPG PSRRKRQPSM 840
SETMPLYTLC KEDLESMDKE VDDILGEGSD DSDSEKRRPE EQEEEPQPRK PGTRRERTLG 900
APASSERSAA GGRGPRGHKR KLNEEDAASE SSRESSNEDE GSSSEADEMA KALEAELNDL 960
M 961 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IDA:HPA.
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0030496; C:midbody; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0051233; C:spindle midzone; IDA:UniProtKB.
 GO:0000922; C:spindle pole; IDA:UniProtKB.
 GO:0008420; F:CTD phosphatase activity; IDA:UniProtKB.
 GO:0003899; F:DNA-directed RNA polymerase activity; TAS:ProtInc.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0010458; P:exit from mitosis; IMP:UniProtKB.
 GO:0050434; P:positive regulation of viral transcription; TAS:Reactome.
 GO:0006470; P:protein dephosphorylation; IDA:UniProtKB.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 
Interpro
 IPR001357; BRCT_dom.
 IPR015388; FCP1_C.
 IPR011947; FCP1_euk.
 IPR023214; HAD-like_dom.
 IPR004274; NIF. 
Pfam
 PF09309; FCP1_C
 PF03031; NIF
 PF12738; PTCB-BRCT 
SMART
 SM00292; BRCT
 SM00577; CPDc 
PROSITE
 PS50172; BRCT
 PS50969; FCP1 
PRINTS