UniProt Accession

 RU2B_HUMAN; P08579; B2R7J3; D3DW21; Q9UJD4 

Genbank Protein ID

 M15841; AK313004; AL034428; CH471133; CH471133; BC018022; BC036737 

Genbank Nucleotide ID

 AAA36796.1; BAG35840.1; CAB38777.2; EAX10284.1; EAX10285.1; AAH18022.1; AAH36737.1 

Protein Name

 U2 small nuclear ribonucleoprotein B'' 

Protein Synonyms/Alias

 U2 snRNP B'' 

Gene Name

 SNRPB2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
17	YINNMNDKIKKEELK	ubiquitination	[1]
57	GQAFVIFKELGSSTN	ubiquitination	[1, 2, 3, 4, 5]
77	QGFPFYGKPMRIQYA	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
85	PMRIQYAKTDSDIIS	ubiquitination	[1, 2, 4, 5, 6, 7]
93	TDSDIISKMRGTFAD	ubiquitination	[1, 2, 4, 5, 6, 7]
111	KKEKKKAKTVEQTAT	ubiquitination	[6]
219	ITPSHAMKITYAKK*	ubiquitination	[2, 3, 5]

Reference

 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Involved in pre-mRNA splicing. This protein is associated with snRNP U2. It binds stem loop IV of U2 snRNA only in presence of the U2A' protein. 

Sequence Annotation

 DOMAIN 7 86 RRM 1.
 DOMAIN 151 225 RRM 2.
 MOD_RES 151 151 Phosphotyrosine.  

Keyword

 3D-structure; Complete proteome; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Ribonucleoprotein; RNA-binding; Spliceosome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 225 AA 

Protein Sequence

Gene Ontology

 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005686; C:U2 snRNP; TAS:ProtInc.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0017069; F:snRNA binding; IEA:InterPro.
 GO:0000398; P:mRNA splicing, via spliceosome; IC:UniProtKB. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR024888; U1_snRNP_A/U2_snRNP_B''. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS