UniProt Accession

 DHAS_ECOLI; P0A9Q9; P00353; Q2M797 

Genbank Protein ID

 V00262; U18997; U00096; AP009048 

Genbank Nucleotide ID

 CAA23511.1; AAA58231.1; AAC76458.1; BAE77859.1 

Protein Name

 Aspartate-semialdehyde dehydrogenase 

Protein Synonyms/Alias

 ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase 

Gene Name

 asd 

Gene Synonyms/Alias

 hom; b3433; JW3396 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
84	YTNEIYPKLRESGWQ	acetylation	[1]
104	AASSLRMKDDAIIIL	acetylation	[1]
232	SLIPWIDKQLDNGQS	acetylation	[1, 2]
244	GQSREEWKGQAETNK	acetylation	[1]
281	HSQAFTIKLKKDVSI	acetylation	[1]
283	QAFTIKLKKDVSIPT	acetylation	[1]
284	AFTIKLKKDVSIPTV	acetylation	[3]
303	AAHNPWAKVVPNDRE	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508]
 [3] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L- aspartyl-4-phosphate. 

Sequence Annotation

 NP_BIND 10 13 NADP.
 NP_BIND 37 38 NADP.
 NP_BIND 165 169 NADP.
 ACT_SITE 135 135 Acyl-thioester intermediate.
 ACT_SITE 274 274 Proton acceptor.
 BINDING 73 73 NADP.
 BINDING 102 102 Phosphate (By similarity).
 BINDING 162 162 Substrate.
 BINDING 173 173 NADP.
 BINDING 193 193 NADP; via carbonyl oxygen.
 BINDING 241 241 Substrate.
 BINDING 244 244 Phosphate (By similarity).
 BINDING 267 267 Substrate.
 BINDING 350 350 NADP.
 MOD_RES 135 135 S-cysteinyl cysteine; in inhibited form.  

Keyword

 3D-structure; Amino-acid biosynthesis; Complete proteome; Diaminopimelate biosynthesis; Direct protein sequencing; Lysine biosynthesis; Methionine biosynthesis; NADP; Oxidoreductase; Reference proteome; Threonine biosynthesis. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 367 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0004073; F:aspartate-semialdehyde dehydrogenase activity; IDA:EcoCyc.
 GO:0003942; F:N-acetyl-gamma-glutamyl-phosphate reductase activity; IEA:InterPro.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0050661; F:NADP binding; IEA:HAMAP.
 GO:0071266; P:'de novo' L-methionine biosynthetic process; IEA:HAMAP.
 GO:0019877; P:diaminopimelate biosynthetic process; IEA:HAMAP.
 GO:0009090; P:homoserine biosynthetic process; IDA:EcoCyc.
 GO:0009097; P:isoleucine biosynthetic process; IEA:InterPro.
 GO:0009089; P:lysine biosynthetic process via diaminopimelate; IDA:EcoCyc.
 GO:0006974; P:response to DNA damage stimulus; IEP:EcoliWiki.
 GO:0009088; P:threonine biosynthetic process; IEA:HAMAP. 

Interpro

 IPR000319; Asp-semialdehyde_DH_CS.
 IPR011534; Asp_ADH_gamma-type.
 IPR012080; Asp_semialdehyde_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR000534; Semialdehyde_DH_NAD-bd.
 IPR012280; Semialdhyde_DH_dimer_dom. 

Pfam

 PF01118; Semialdhyde_dh
 PF02774; Semialdhyde_dhC 

SMART

 SM00859; Semialdhyde_dh 

PROSITE

 PS01103; ASD 

PRINTS