UniProt Accession

 ENO1_YEAST; P00924; D6VV34; P99013 

Genbank Protein ID

 J01322; X99228; Z73039; BK006941 

Genbank Nucleotide ID

 AAA88712.1; CAA67616.1; CAA97283.1; DAA08345.1 

Protein Name

 Enolase 1 

Protein Synonyms/Alias

 2-phospho-D-glycerate hydro-lyase 1; 2-phosphoglycerate dehydratase 1 

Gene Name

 ENO1 

Gene Synonyms/Alias

 ENOA; HSP48; YGR254W; G9160 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
5	***MAVSKVYARSVY	acetylation	[1]
28	EVELTTEKGVFRSIV	acetylation	[1]
60	DKSKWMGKGVLHAVK	acetylation	[1]
60	DKSKWMGKGVLHAVK	ubiquitination	[2]
67	KGVLHAVKNVNDVIA	acetylation	[1]
79	VIAPAFVKANIDVKD	acetylation	[1]
85	VKANIDVKDQKAVDD	ubiquitination	[2]
88	NIDVKDQKAVDDFLI	ubiquitination	[2]
126	SRAAAAEKNVPLYKH	acetylation	[1]
132	EKNVPLYKHLADLSK	acetylation	[1]
132	EKNVPLYKHLADLSK	ubiquitination	[2]
139	KHLADLSKSKTSPYV	acetylation	[1]
195	SEVYHNLKSLTKKRY	acetylation	[1]
195	SEVYHNLKSLTKKRY	ubiquitination	[2, 3]
234	DLIVDAIKAAGHDGK	ubiquitination	[2]
255	CASSEFFKDGKYDLD	acetylation	[1]
255	CASSEFFKDGKYDLD	ubiquitination	[2]
258	SEFFKDGKYDLDFKN	acetylation	[1]
264	GKYDLDFKNPNSDKS	acetylation	[1]
329	DLTVTNPKRIATAIE	acetylation	[1]
337	RIATAIEKKAADALL	acetylation	[1]
337	RIATAIEKKAADALL	ubiquitination	[2]
338	IATAIEKKAADALLL	acetylation	[1]
338	IATAIEKKAADALLL	ubiquitination	[2]
346	AADALLLKVNQIGTL	acetylation	[1]
346	AADALLLKVNQIGTL	ubiquitination	[2, 3]
358	GTLSESIKAAQDSFA	ubiquitination	[2, 3]
397	GLRTGQIKTGAPARS	acetylation	[1]
397	GLRTGQIKTGAPARS	ubiquitination	[2]
409	ARSERLAKLNQLLRI	acetylation	[1]
409	ARSERLAKLNQLLRI	ubiquitination	[2]
436	ENFHHGDKL******	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

  

Sequence Annotation

 REGION 373 376 Substrate binding.
 ACT_SITE 212 212 Proton donor (Probable).
 ACT_SITE 346 346 Proton acceptor.
 METAL 247 247 Magnesium.
 METAL 296 296 Magnesium.
 METAL 321 321 Magnesium.
 BINDING 160 160 Substrate.
 BINDING 169 169 Substrate.
 BINDING 296 296 Substrate.
 BINDING 321 321 Substrate.
 BINDING 397 397 Substrate.
 MOD_RES 96 96 Phosphoserine.
 MOD_RES 104 104 Phosphoserine.
 MOD_RES 119 119 Phosphoserine.
 MOD_RES 138 138 Phosphoserine (By similarity).
 MOD_RES 188 188 Phosphoserine (By similarity).
 MOD_RES 313 313 Phosphothreonine (By similarity).
 MOD_RES 324 324 Phosphothreonine (By similarity).  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Lyase; Magnesium; Metal-binding; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 437 AA 

Protein Sequence

Gene Ontology

 GO:0000324; C:fungal-type vacuole; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0000015; C:phosphopyruvate hydratase complex; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0004634; F:phosphopyruvate hydratase activity; IMP:SGD.
 GO:0006094; P:gluconeogenesis; IEP:SGD.
 GO:0006096; P:glycolysis; IMP:SGD.
 GO:0032889; P:regulation of vacuole fusion, non-autophagic; IDA:SGD. 

Interpro

 IPR000941; Enolase.
 IPR020810; Enolase_C.
 IPR020809; Enolase_CS.
 IPR020811; Enolase_N. 

Pfam

 PF00113; Enolase_C
 PF03952; Enolase_N 

SMART

  

PROSITE

 PS00164; ENOLASE 

PRINTS

 PR00148; ENOLASE.