UniProt Accession

 A6NC17_HUMAN; A6NC17 

Genbank Protein ID

 AC022506 

Genbank Nucleotide ID

  

Protein Name

 Methionine--tRNA ligase, cytoplasmic 

Protein Synonyms/Alias

  

Gene Name

 MARS 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
101	TPQEICDKYHIIHAD	ubiquitination	[1]
130	TTTPQQTKITQDIFQ	ubiquitination	[1, 2]
141	DIFQQLLKRGFVLQD	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
186	ARGDQCDKCGKLINA	ubiquitination	[5]
189	DQCDKCGKLINAVEL	ubiquitination	[1]
197	LINAVELKKPQCKVC	ubiquitination	[1, 7]
266	RCITRDLKWGTPVPL	ubiquitination	[2, 3, 6]
429	RAGMFVSKFFGGYVP	ubiquitination	[2, 6, 8]
464	HYHQLLEKVRIRDAL	ubiquitination	[8]
492	IQVNEPWKRIKGSEA	acetylation	[7]
492	IQVNEPWKRIKGSEA	ubiquitination	[1, 2, 5, 6]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 635 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004825; F:methionine-tRNA ligase activity; IEA:InterPro.
 GO:0006431; P:methionyl-tRNA aminoacylation; IEA:InterPro. 

Interpro

 IPR001412; aa-tRNA-synth_I_CS.
 IPR023458; Met-tRNA_ligase_1.
 IPR014758; Met-tRNA_synth.
 IPR015413; Methionyl/Leucyl_tRNA_Synth.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009080; tRNAsynth_1a_anticodon-bd. 

Pfam

 PF09334; tRNA-synt_1g 

SMART

  

PROSITE

 PS00178; AA_TRNA_LIGASE_I 

PRINTS

 PR01041; TRNASYNTHMET.