CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005076
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tyrosine--tRNA ligase 1 
Protein Synonyms/Alias
 Tyrosyl-tRNA synthetase 1; TyrRS 1 
Gene Name
 tyrS1 
Gene Synonyms/Alias
 BSU29670 
Created Date
 July 27, 2013 
Organism
 Bacillus subtilis (strain 168) 
NCBI Taxa ID
 224308 
Lysine Modification
Position
Peptide
Type
References
235ADGTKFGKTEGGAIWacetylation[1]
Reference
 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 
Functional Description
 Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) (By similarity). 
Sequence Annotation
 DOMAIN 355 421 S4 RNA-binding.
 MOTIF 40 49 "HIGH" region.
 MOTIF 232 236 "KMSKS" region.
 BINDING 35 35 Tyrosine (By similarity).
 BINDING 170 170 Tyrosine (By similarity).
 BINDING 174 174 Tyrosine (By similarity).
 BINDING 235 235 ATP (By similarity).  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 422 AA 
Protein Sequence
MTNLLEDLSF RGLIQQMTDE EGLNKQLNEE KIRLYSGFDP TADSLHIGHL LPILTLRRFQ 60
LAGHHPIALV GGATGLIGDP SGKKAERTLN TADIVSEWSQ KIKNQLSRFL DFEAAENPAV 120
IANNFDWIGK MNVIDFLRDV GKNFGINYML AKDTVSSRIE SGISYTEFSY MILQSYDFLN 180
LYRDKNCKLQ IGGSDQWGNI TAGLELIRKS EEEGAKAFGL TIPLVTKADG TKFGKTEGGA 240
IWLDKEKTSP YEFYQFWINT DDRDVVKYLK YFTFLSKEEI EAYAEKTETA PEKREAQKRL 300
AEEVTSLVHG REALEQAINI SQALFSGNIK ELSAQDVKVG FKDVPSMEVD STQELSLVDV 360
LVQSKLSPSK RQAREDIQNG AVYINGERQT EINYTLSGED RIENQFTVLR RGKKKYFLVT 420
YK 422 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0004831; F:tyrosine-tRNA ligase activity; IEA:HAMAP.
 GO:0006437; P:tyrosyl-tRNA aminoacylation; IEA:HAMAP. 
Interpro
 IPR001412; aa-tRNA-synth_I_CS.
 IPR002305; aa-tRNA-synth_Ic.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR002942; S4_RNA-bd.
 IPR002307; Tyr-tRNA-ligase.
 IPR024088; Tyr-tRNA-ligase_bac-type.
 IPR024107; Tyr-tRNA-ligase_bac_1. 
Pfam
 PF01479; S4
 PF00579; tRNA-synt_1b 
SMART
 SM00363; S4 
PROSITE
 PS00178; AA_TRNA_LIGASE_I
 PS50889; S4 
PRINTS
 PR01040; TRNASYNTHTYR.