UniProt Accession

 HIGB_ECOLI; P64578; P42595; Q2M9C3 

Genbank Protein ID

 U18997; U00096; AP009048 

Genbank Nucleotide ID

 AAA57884.1; AAC76118.1; BAE77133.1 

Protein Name

 mRNA interferase HigB 

Protein Synonyms/Alias

 Endoribonuclease HigB; Toxin HigB 

Gene Name

 higB 

Gene Synonyms/Alias

 ygjN; b3083; JW3054 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
52	FPSLDNFKYLDKHYV	acetylation	[1]
56	DNFKYLDKHYVFNVG	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Toxic component of a toxin-antitoxin (TA) module. A probable translation-dependent mRNA interferase. Overexpression causes cessation of cell growth and inhibits cell proliferation via inhibition of translation; this blockage is overcome by subsequent expression of antitoxin HigA. Overexpression causes cleavage of a number of mRNAs in a translation-dependent fashion, suggesting this is an mRNA interferase. 

Sequence Annotation

  

Keyword

 Complete proteome; Endonuclease; Hydrolase; Nuclease; Reference proteome; RNA-binding; Stress response; Toxin. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 104 AA 

Protein Sequence

Gene Ontology

 GO:0004521; F:endoribonuclease activity; IDA:EcoCyc.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0017148; P:negative regulation of translation; IDA:EcoCyc.
 GO:0043488; P:regulation of mRNA stability; IDA:EcoCyc.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW. 

Interpro

 IPR018669; Toxin_HigB_put. 

Pfam

 PF09907; DUF2136 

SMART

  

PROSITE

  

PRINTS