CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012438
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 N-alpha-acetyltransferase 25, NatB auxiliary subunit 
Protein Synonyms/Alias
 Mitochondrial distribution and morphology protein 20; N-terminal acetyltransferase B complex subunit MDM20; NatB complex subunit MDM20; N-terminal acetyltransferase B complex subunit NAA25; p120 
Gene Name
 NAA25 
Gene Synonyms/Alias
 C12orf30; MDM20; NAP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
108LYEAAVKKVPNSEEYubiquitination[1]
183LAERMVEKMVKEDKIubiquitination[2]
208MILERLGKYQEALDVubiquitination[1]
219ALDVIRGKLGEKLTSmethylation[3]
223IRGKLGEKLTSEIQSubiquitination[1, 4]
240NKCMAMYKKLSRWPEubiquitination[1]
304YSAEKAVKFIEDRITubiquitination[1]
315DRITEESKSSRHLRGubiquitination[4]
327LRGPHLAKLELIRRLubiquitination[1, 5, 6]
344QGCNDEYKLGDPEELubiquitination[7]
357ELMFQYFKKFGDKPCubiquitination[2, 4, 5, 6]
358LMFQYFKKFGDKPCCubiquitination[1]
401LSTPTEDKLALPADIubiquitination[1, 4, 7]
435HTMDKNQKLSVVRELubiquitination[1]
575RFFHSNQKDTSEYIIubiquitination[1, 4]
586EYIIQAYKYGAFEKIubiquitination[4]
592YKYGAFEKIPEFIAFubiquitination[1, 4]
676RDVSEEHKKLSLEEEubiquitination[4]
714VEPKNSEKTAENGVSubiquitination[1, 8]
742EATLETGKRFIEKDIubiquitination[1, 2, 4, 7]
747TGKRFIEKDIQYPFLubiquitination[1, 2, 4]
806ERIENSFKSLLDQLKubiquitination[4]
813KSLLDQLKDVFSKCKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Non-catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp-Glu. May play a role in normal cell-cycle progression. 
Sequence Annotation
 REPEAT 11 44 TPR 1.
 REPEAT 45 78 TPR 2.
 REPEAT 79 112 TPR 3.
 REPEAT 114 146 TPR 4.
 MOD_RES 691 691 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Phosphoprotein; Polymorphism; Reference proteome; Repeat; TPR repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 972 AA 
Protein Sequence
MATRGHVQDP NDRRLRPIYD YLDNGNNKMA IQQADKLLKK HKDLHCAKVL KAIGLQRTGK 60
QEEAFTLAQE VAALEPTDDN SLQALTILYR EMHRPELVTK LYEAAVKKVP NSEEYHSHLF 120
MAYARVGEYK KMQQAGMALY KIVPKNPYYF WSVMSLIMQS ISAQDENLSK TMFLPLAERM 180
VEKMVKEDKI EAEAEVELYY MILERLGKYQ EALDVIRGKL GEKLTSEIQS RENKCMAMYK 240
KLSRWPECNA LSRRLLLKNS DDWQFYLTYF DSVFRLIEEA WSPPAEGEHS LEGEVHYSAE 300
KAVKFIEDRI TEESKSSRHL RGPHLAKLEL IRRLRSQGCN DEYKLGDPEE LMFQYFKKFG 360
DKPCCFTDLK VFVDLLPATQ CTKFINQLLG VVPLSTPTED KLALPADIRA LQQHLCVVQL 420
TRLLGLYHTM DKNQKLSVVR ELMLRYQHGL EFGKTCLKTE LQFSDYYCLL AVHALIDVWR 480
ETGDETTVWQ ALTLLEEGLT HSPSNAQFKL LLVRIYCMLG AFEPVVDLYS SLDAKHIQHD 540
TIGYLLTRYA ESLGQYAAAS QSCNFALRFF HSNQKDTSEY IIQAYKYGAF EKIPEFIAFR 600
NRLNNSLHFA QVRTERMLLD LLLEANISTS LAESIKSMNL RPEEDDIPWE DLRDNRDLNV 660
FFSWDPKDRD VSEEHKKLSL EEETLWLRIR SLTLRLISGL PSLNHPVEPK NSEKTAENGV 720
SSRIDILRLL LQQLEATLET GKRFIEKDIQ YPFLGPVPTR MGGFFNSGCS QCQISSFYLV 780
NDIYELDTSG LEDTMEIQER IENSFKSLLD QLKDVFSKCK GDLLEVKDGN LKTHPTLLEN 840
LVFFVETISV ILWVSSYCES VLRPYKLNLQ KKKKKKKETS IIMPPVFTSF QDYVTGLQTL 900
ISNVVDHIKG LETHLIALKL EELILEDTSL SPEERKFSKT VQGKVQSSYL HSLLEMGELL 960
KKRLETTKKL KI 972 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. 
Interpro
 IPR019183; N-acetylTrfase_B_cplx_non-cat.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical. 
Pfam
 PF09797; NatB_MDM20 
SMART
  
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS