CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000678
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transforming growth factor beta-1-induced transcript 1 protein 
Protein Synonyms/Alias
 Androgen receptor coactivator 55 kDa protein; Androgen receptor-associated protein of 55 kDa; Hydrogen peroxide-inducible clone 5 protein; Hic-5 
Gene Name
 TGFB1I1 
Gene Synonyms/Alias
 ARA55 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
102MSQFPSSKVASGEQKubiquitination[1]
109KVASGEQKEDQSEDKubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA- binding activity. 
Sequence Annotation
 DOMAIN 226 285 LIM zinc-binding 1.
 DOMAIN 286 343 LIM zinc-binding 2.
 DOMAIN 344 403 LIM zinc-binding 3.
 DOMAIN 404 461 LIM zinc-binding 4.
 REGION 1 240 Interaction with PTK2B/PYK2.
 REGION 1 200 Transcription activation (By similarity).
 REGION 83 136 Interaction with PTK2/FAK1 (By
 MOTIF 3 15 LD motif 1.
 MOTIF 92 104 LD motif 2.
 MOTIF 157 168 LD motif 3.
 MOTIF 203 215 LD motif 4.
 MOD_RES 33 33 Phosphothreonine.
 MOD_RES 38 38 Phosphotyrosine (By similarity).
 MOD_RES 60 60 Phosphotyrosine; by FAK2 and FYN.
 MOD_RES 68 68 Phosphoserine.
 MOD_RES 137 137 Phosphoserine.  
Keyword
 Activator; Alternative splicing; Cell junction; Complete proteome; Cytoplasm; Cytoskeleton; Differentiation; LIM domain; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Wnt signaling pathway; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 461 AA 
Protein Sequence
MPRSGAPKER PAEPLTPPPS YGHQPQTGSG ESSGASGDKD HLYSTVCKPR SPKPAAPAAP 60
PFSSSSGVLG TGLCELDRLL QELNATQFNI TDEIMSQFPS SKVASGEQKE DQSEDKKRPS 120
LPSSPSPGLP KASATSATLE LDRLMASLSD FRVQNHLPAS GPTQPPVVSS TNEGSPSPPE 180
PTGKGSLDTM LGLLQSDLSR RGVPTQAKGL CGSCNKPIAG QVVTALGRAW HPEHFVCGGC 240
STALGGSSFF EKDGAPFCPE CYFERFSPRC GFCNQPIRHK MVTALGTHWH PEHFCCVSCG 300
EPFGDEGFHE REGRPYCRRD FLQLFAPRCQ GCQGPILDNY ISALSALWHP DCFVCRECFA 360
PFSGGSFFEH EGRPLCENHF HARRGSLCAT CGLPVTGRCV SALGRRFHPD HFTCTFCLRP 420
LTKGSFQERA GKPYCQPCFL KLFG 444 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005925; C:focal adhesion; IEA:UniProtKB-SubCell.
 GO:0005622; C:intracellular; IDA:UniProtKB.
 GO:0016363; C:nuclear matrix; IEA:UniProtKB-SubCell.
 GO:0050681; F:androgen receptor binding; NAS:UniProtKB.
 GO:0003713; F:transcription coactivator activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0030521; P:androgen receptor signaling pathway; NAS:UniProtKB.
 GO:0007155; P:cell adhesion; TAS:ProtInc.
 GO:0045165; P:cell fate commitment; IEA:Compara.
 GO:0030855; P:epithelial cell differentiation; IEA:Compara.
 GO:0016331; P:morphogenesis of embryonic epithelium; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; TAS:ProtInc.
 GO:0045599; P:negative regulation of fat cell differentiation; IEA:Compara.
 GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IDA:BHF-UCL.
 GO:0010718; P:positive regulation of epithelial to mesenchymal transition; IDA:BHF-UCL.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0030511; P:positive regulation of transforming growth factor beta receptor signaling pathway; IDA:BHF-UCL.
 GO:0009408; P:response to heat; IEA:Compara.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0030579; P:ubiquitin-dependent SMAD protein catabolic process; IDA:BHF-UCL.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 
Interpro
 IPR017305; Leupaxin.
 IPR001781; Znf_LIM. 
Pfam
 PF00412; LIM 
SMART
 SM00132; LIM 
PROSITE
 PS00478; LIM_DOMAIN_1
 PS50023; LIM_DOMAIN_2 
PRINTS