UniProt Accession

 NUOE_ECOLI; P0AFD1; P33601 

Genbank Protein ID

 X68301; L25055; U00096; AP009048 

Genbank Nucleotide ID

 CAA48364.1; AAA03536.1; AAC75345.1; BAA16114.1 

Protein Name

 NADH-quinone oxidoreductase subunit E 

Protein Synonyms/Alias

 NADH dehydrogenase I subunit E; NDH-1 subunit E; NUO5 

Gene Name

 nuoE 

Gene Synonyms/Alias

 b2285; JW2280 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
41	AASIEALKIVQKQRG	acetylation	[1]
111	GIQAALEKKLNIKPG	acetylation	[1, 2]
112	IQAALEKKLNIKPGQ	acetylation	[1]
116	LEKKLNIKPGQTTFD	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. 

Sequence Annotation

 METAL 92 92 Iron-sulfur (2Fe-2S) (Potential).
 METAL 97 97 Iron-sulfur (2Fe-2S) (Potential).
 METAL 133 133 Iron-sulfur (2Fe-2S) (Potential).
 METAL 137 137 Iron-sulfur (2Fe-2S) (Potential).  

Keyword

 2Fe-2S; Complete proteome; Direct protein sequencing; Iron; Iron-sulfur; Metal-binding; NAD; Oxidoreductase; Quinone; Reference proteome; Ubiquinone. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 166 AA 

Protein Sequence

Gene Ontology

 GO:0005747; C:mitochondrial respiratory chain complex I; IBA:RefGenome.
 GO:0045272; C:plasma membrane respiratory chain complex I; IDA:EcoCyc.
 GO:0051537; F:2 iron, 2 sulfur cluster binding; ISS:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IBA:RefGenome.
 GO:0048038; F:quinone binding; IEA:UniProtKB-KW.
 GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IBA:RefGenome. 

Interpro

 IPR002023; 2Fe-2S_Ferredox.
 IPR012336; Thioredoxin-like_fold. 

Pfam

 PF01257; Complex1_24kDa 

SMART

  

PROSITE

 PS01099; COMPLEX1_24K 

PRINTS