CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013002
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 GMP synthase [glutamine-hydrolyzing] 
Protein Synonyms/Alias
 GMP synthetase; Glutamine amidotransferase 
Gene Name
 Gmps 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
145SLFRGLQKEEIVLLTacetylation[1]
145SLFRGLQKEEIVLLTubiquitination[2]
165DKVADGFKVVARSGNacetylation[1]
165DKVADGFKVVARSGNubiquitination[2]
182AGIANESKKLYGVQFubiquitination[2]
183GIANESKKLYGVQFHubiquitination[2]
204ENGKVILKNFLYDIAacetylation[1]
277IDNGFMRKRESQSVEubiquitination[2]
288QSVEEALKKLGIQVKubiquitination[2]
412GKVIEPLKDFHKDEVubiquitination[2]
457AEEPYICKDFPETNNubiquitination[2]
477ADFSASVKKPHTLLQubiquitination[2]
478DFSASVKKPHTLLQRubiquitination[2]
487HTLLQRVKACTTEEDubiquitination[2]
627FDRDPLQKQPSCQRSubiquitination[2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division (By similarity). 
Sequence Annotation
 DOMAIN 27 216 Glutamine amidotransferase type-1.
 DOMAIN 217 435 GMPS ATP-PPase.
 NP_BIND 244 250 ATP (By similarity).
 ACT_SITE 104 104 For GATase activity (By similarity).
 ACT_SITE 190 190 For GATase activity (By similarity).
 ACT_SITE 192 192 For GATase activity (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 9 9 N6-acetyllysine (By similarity).
 MOD_RES 318 318 Phosphothreonine (By similarity).
 MOD_RES 332 332 Phosphoserine.  
Keyword
 Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glutamine amidotransferase; GMP biosynthesis; Ligase; Nucleotide-binding; Phosphoprotein; Purine biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 693 AA 
Protein Sequence
MALCNGDSKP ENAGGDLKDG SHHYEGAVVI LDAGAQYGKV IDRRVRELFV QSEIFPLETP 60
AFAIKEQGFR AIIISGGPNS VYAEDAPWFD PAIFTIGKPI LGICYGMQMM NKVFGGTVHK 120
KSVREDGVFN ISMDNTCSLF RGLQKEEIVL LTHGDSVDKV ADGFKVVARS GNIVAGIANE 180
SKKLYGVQFH PEVGLTENGK VILKNFLYDI AGCSGNFTVQ NRELECIREI KEKVGTSKVL 240
VLLSGGVDST VCTALLNRAL NQDQVIAVHI DNGFMRKRES QSVEEALKKL GIQVKVINAA 300
HSFYNGTTTL PISDEDRTPR KRISKTLNMT TSPEEKRKII GDTFVKIANE VIGEMSLKPE 360
EVFLAQGTLR PDLIESASLV ASGKAELIKT HHNDTELIRK LREEGKVIEP LKDFHKDEVR 420
ILGRELDLPE ELVSRHPFPG PGLAIRVICA EEPYICKDFP ETNNILKIVA DFSASVKKPH 480
TLLQRVKACT TEEDQEKLMQ ITSLHSLNAF LLPIKTVGVQ GDCRSYSYVC GISSKDEPDW 540
ESLIFLARLI PRMCHNINRV VYIFGPPVKE PPTDVTPTFL TTGVLSTLRQ ADFEAHNILR 600
ESGFAGKISQ MPVILTPLHF DRDPLQKQPS CQRSVVIRTF ITSDFMTGVP ATPGNEIPVE 660
VVLKMVTEIK KIPGISRIMY DLTSKPPGTT EWE 693 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003922; F:GMP synthase (glutamine-hydrolyzing) activity; IEA:EC.
 GO:0016462; F:pyrophosphatase activity; IEA:InterPro.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
 GO:0006177; P:GMP biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR017926; GATASE.
 IPR001674; GMP_synth_C.
 IPR004739; GMP_synth_N.
 IPR025777; GMPS_ATP_PPase_dom.
 IPR022310; NAD/GMP_synthase.
 IPR014729; Rossmann-like_a/b/a_fold. 
Pfam
 PF00117; GATase
 PF00958; GMP_synt_C
 PF02540; NAD_synthase 
SMART
  
PROSITE
 PS51273; GATASE_TYPE_1
 PS51553; GMPS_ATP_PPASE 
PRINTS