CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019811
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein arginine N-methyltransferase 1 
Protein Synonyms/Alias
 Histone-arginine N-methyltransferase PRMT1; Interferon receptor 1-bound protein 4 
Gene Name
 PRMT1 
Gene Synonyms/Alias
 HMT2; HRMT1L2; IR1B4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
124VKIVKANKLDHVVTIubiquitination[1, 2]
145EVELPVEKVDIIISEacetylation[3]
173TVLYARDKWLAPDGLubiquitination[4]
223CIKDVAIKEPLVDVVubiquitination[2]
233LVDVVDPKQLVTNACubiquitination[1]
243VTNACLIKEVDIYTVubiquitination[1]
332IGMRPNAKNNRDLDFubiquitination[1, 2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional acivity. 
Sequence Annotation
 ACT_SITE 152 152 By similarity.
 ACT_SITE 161 161 By similarity.
 BINDING 53 53 S-adenosyl-L-methionine (By similarity).
 BINDING 62 62 S-adenosyl-L-methionine (By similarity).
 BINDING 86 86 S-adenosyl-L-methionine; via carbonyl
 BINDING 108 108 S-adenosyl-L-methionine (By similarity).
 BINDING 137 137 S-adenosyl-L-methionine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Methyltransferase; Nucleus; Polymorphism; Reference proteome; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 361 AA 
Protein Sequence
MENFVATLAN GMSLQPPLEE VSCGQAESSE KPNAEDMTSK DYYFDSYAHF GIHEEMLKDE 60
VRTLTYRNSM FHNRHLFKDK VVLDVGSGTG ILCMFAAKAG ARKVIGIECS SISDYAVKIV 120
KANKLDHVVT IIKGKVEEVE LPVEKVDIII SEWMGYCLFY ESMLNTVLYA RDKWLAPDGL 180
IFPDRATLYV TAIEDRQYKD YKIHWWENVY GFDMSCIKDV AIKEPLVDVV DPKQLVTNAC 240
LIKEVDIYTV KVEDLTFTSP FCLQVKRNDY VHALVAYFNI EFTRCHKRTG FSTSPESPYT 300
HWKQTVFYME DYLTVKTGEE IFGTIGMRPN AKNNRDLDFT IDLDFKGQLC ELSCSTDYRM 360
R 361 
Gene Ontology
 GO:0005737; C:cytoplasm; TAS:ProtInc.
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0016275; F:[cytochrome c]-arginine N-methyltransferase activity; IEA:Compara.
 GO:0044020; F:histone methyltransferase activity (H4-R3 specific); IDA:UniProtKB.
 GO:0035242; F:protein-arginine omega-N asymmetric methyltransferase activity; IEA:Compara.
 GO:0035241; F:protein-arginine omega-N monomethyltransferase activity; IEA:Compara.
 GO:0030519; F:snoRNP binding; IEA:Compara.
 GO:0007166; P:cell surface receptor signaling pathway; TAS:ProtInc.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0045653; P:negative regulation of megakaryocyte differentiation; IDA:UniProtKB.
 GO:0031175; P:neuron projection development; IMP:UniProtKB. 
Interpro
 IPR025799; Arg_MeTrfase.
 IPR025714; Methyltranfer_dom. 
Pfam
 PF13847; Methyltransf_31 
SMART
  
PROSITE
  
PRINTS