CPLM-022836

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-022836

UniProt Accession

MTRR_HUMAN; Q9UBK8; O60471; Q32MA9; Q7Z4M8

Genbank Protein ID

AF121213; AF121202; AF121203; AF121204; AF121205; AF121206; AF121207; AF121208; AF121209; AF121210; AF121211; AF121212; AF121214; AF121213; AF121202; AF121203; AF121204; AF121205; AF121206; AF121207; AF121208; AF121209; AF121210; AF121211; AF121212; AF025794; AC010346; AC025174; BC054816; BC109216

Genbank Nucleotide ID

AAF17303.1; AAF17303.1; AAF17303.1; AAF17303.1; AAF17303.1; AAF17303.1; AAF17303.1; AAF17303.1; AAF17303.1; AAF17303.1; AAF17303.1; AAF17303.1; AAF16876.1; AAF17304.1; AAF17304.1; AAF17304.1; AAF17304.1; AAF17304.1; AAF17304.1; AAF17304.1; AAF17304.1; AAF17304.1; AAF17304.1; AAF17304.1; AAF17304.1; AAC39667.1; AAH54816.2; AAI09217.1

Protein Name

Methionine synthase reductase

Protein Synonyms/Alias

MSR

Gene Name

MTRR

Gene Synonyms/Alias

Created Date

July 27, 2013

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Lysine Modification

Position	Peptide	Type	References
101	DTARKFVKEIQNQTL	ubiquitination	[1]
208	SSRTDLVKSELLHIE	ubiquitination	[2]
434	RLQELCSKQGAADYS	ubiquitination	[1, 3]
650	GEEEAPAKYVQDNIQ	ubiquitination	[1]

Reference

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Involved in the reductive regeneration of cob(I)alamin cofactor required for the maintenance of methionine synthase in a functional state.

Sequence Annotation

DOMAIN 32 174 Flavodoxin-like.
DOMAIN 298 560 FAD-binding FR-type.
NP_BIND 120 151 FMN (By similarity).
NP_BIND 478 481 FAD.
NP_BIND 514 517 FAD.
NP_BIND 637 638 NADP.
NP_BIND 651 653 NADP.
REGION 193 274 Hinge.
BINDING 318 318 NADP.
BINDING 686 686 NADP.
BINDING 724 724 FAD.
MOD_RES 198 198 Phosphoserine.

Keyword

3D-structure; Alternative splicing; Amino-acid biosynthesis; Complete proteome; Cytoplasm; Disease mutation; FAD; Flavoprotein; FMN; Methionine biosynthesis; NADP; Oxidoreductase; Phosphoprotein; Polymorphism; Reference proteome.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

725 AA

Protein Sequence

MGAASVRAGA RLVEVALCSF TVTCLEVMRR FLLLYATQQG QAKAIAEEIC EQAVVHGFSA 60
DLHCISESDK YDLKTETAPL VVVVSTTGTG DPPDTARKFV KEIQNQTLPV DFFAHLRYGL 120
LGLGDSEYTY FCNGGKIIDK RLQELGARHF YDTGHADDCV GLELVVEPWI AGLWPALRKH 180
FRSSRGQEEI SGALPVASPA SSRTDLVKSE LLHIESQVEL LRFDDSGRKD SEVLKQNAVN 240
SNQSNVVIED FESSLTRSVP PLSQASLNIP GLPPEYLQVH LQESLGQEES QVSVTSADPV 300
FQVPISKAVQ LTTNDAIKTT LLVELDISNT DFSYQPGDAF SVICPNSDSE VQSLLQRLQL 360
EDKREHCVLL KIKADTKKKG ATLPQHIPAG CSLQFIFTWC LEIRAIPKKA FLRALVDYTS 420
DSAEKRRLQE LCSKQGAADY SRFVRDACAC LLDLLLAFPS CQPPLSLLLE HLPKLQPRPY 480
SCASSSLFHP GKLHFVFNIV EFLSTATTEV LRKGVCTGWL ALLVASVLQP NIHASHEDSG 540
KALAPKISIS PRTTNSFHLP DDPSIPIIMV GPGTGIAPFI GFLQHREKLQ EQHPDGNFGA 600
MWLFFGCRHK DRDYLFRKEL RHFLKHGILT HLKVSFSRDA PVGEEEAPAK YVQDNIQLHG 660
QQVARILLQE NGHIYVCGDA KNMAKDVHDA LVQIISKEVG VEKLEAMKTL ATLKEEKRYL 720
QDIWS 725

Gene Ontology

GO:0005829; C:cytosol; TAS:UniProtKB.
GO:0045111; C:intermediate filament cytoskeleton; IDA:HPA.
GO:0005634; C:nucleus; IDA:HPA.
GO:0030586; F:[methionine synthase] reductase activity; TAS:UniProtKB.
GO:0050660; F:flavin adenine dinucleotide binding; IDA:UniProtKB.
GO:0010181; F:FMN binding; TAS:UniProtKB.
GO:0005506; F:iron ion binding; IEA:InterPro.
GO:0050661; F:NADP binding; TAS:UniProtKB.
GO:0016709; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen; IBA:RefGenome.
GO:0034641; P:cellular nitrogen compound metabolic process; TAS:Reactome.
GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
GO:0006555; P:methionine metabolic process; TAS:UniProtKB.
GO:0032259; P:methylation; TAS:Reactome.
GO:0006805; P:xenobiotic metabolic process; TAS:Reactome.

Interpro

IPR003097; FAD-binding_1.
IPR017927; Fd_Rdtase_FAD-bd.
IPR001094; Flavdoxin.
IPR008254; Flavodoxin/NO_synth.
IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173; NADPH_Cyt_P450_Rdtase_dom3.
IPR001433; OxRdtase_FAD/NAD-bd.
IPR017938; Riboflavin_synthase-like_b-brl.

Pfam

PF00667; FAD_binding_1
PF00258; Flavodoxin_1
PF00175; NAD_binding_1

SMART

PROSITE

PS51384; FAD_FR
PS50902; FLAVODOXIN_LIKE

PRINTS

PR00369; FLAVODOXIN.
PR00371; FPNCR.