CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003856
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 L-aspartate oxidase 
Protein Synonyms/Alias
 LASPO; Quinolinate synthase B 
Gene Name
 nadB 
Gene Synonyms/Alias
 nicB; b2574; JW2558 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
170VDLIVSDKIGLPGTRacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the oxidation of L-aspartate to iminoaspartate. 
Sequence Annotation
 NP_BIND 12 26 FAD (Potential).
 ACT_SITE 244 244 By similarity.
 ACT_SITE 263 263 By similarity.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Disulfide bond; FAD; Flavoprotein; Oxidoreductase; Pyridine nucleotide biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 540 AA 
Protein Sequence
MNTLPEHSCD VLIIGSGAAG LSLALRLADQ HQVIVLSKGP VTEGSTFYAQ GGIAAVFDET 60
DSIDSHVEDT LIAGAGICDR HAVEFVASNA RSCVQWLIDQ GVLFDTHIQP NGEESYHLTR 120
EGGHSHRRIL HAADATGREV ETTLVSKALN HPNIRVLERS NAVDLIVSDK IGLPGTRRVV 180
GAWVWNRNKE TVETCHAKAV VLATGGASKV YQYTTNPDIS SGDGIAMAWR AGCRVANLEF 240
NQFHPTALYH PQARNFLLTE ALRGEGAYLK RPDGTRFMPD FDERGELAPR DIVARAIDHE 300
MKRLGADCMF LDISHKPADF IRQHFPMIYE KLLGLGIDLT QEPVPIVPAA HYTCGGVMVD 360
DHGRTDVEGL YAIGEVSYTG LHGANRMASN SLLECLVYGW SAAEDITRRM PYAHDISTLP 420
PWDESRVENP DERVVIQHNW HELRLFMWDY VGIVRTTKRL ERALRRITML QQEIDEYYAH 480
FRVSNNLLEL RNLVQVAELI VRCAMMRKES RGLHFTLDYP ELLTHSGPSI LSPGNHYINR 540 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0050660; F:flavin adenine dinucleotide binding; IDA:EcoCyc.
 GO:0008734; F:L-aspartate oxidase activity; IDA:EcoCyc.
 GO:0044318; F:L-aspartate:fumarate oxidoreductase activity; IDA:EcoCyc.
 GO:0034628; P:de novo NAD biosynthetic process from aspartate; IMP:EcoCyc. 
Interpro
 IPR003953; FAD_bind_dom.
 IPR013027; FAD_pyr_nucl-diS_OxRdtase.
 IPR015939; Fum_Rdtase/Succ_DH_flav-like_C.
 IPR005288; NadB.
 IPR027477; Succ_DH/fumarate_Rdtase_cat. 
Pfam
 PF00890; FAD_binding_2
 PF02910; Succ_DH_flav_C 
SMART
  
PROSITE
  
PRINTS
 PR00368; FADPNR.